摘要
目的使用高效液相色谱法对银蒲解毒片中的秦皮乙素进行含量测定。方法使用色谱柱Inert SustainC18(4.6mm×250mm),以甲醇(B)-0.1%磷酸水溶液(A)(85∶15)作为流动相进行洗脱,检测波长334nm,柱温为30℃,流速1ml/min。结果秦皮乙素在0.256~1.536μg中的线性关系良好,精密度实验RSD=0.688%,线性回归方程为Y=259.81X-193.69,线性范围为0.256~1.536μg,r=0.9999。结论该方法精密度高,准确性好。但秦皮乙素在银蒲解毒片样品含量测定中结果相对偏低,秦皮乙素作为银蒲解毒片质量标准的含量测定项还有待考察。
Objective To determine the content of aesculetin in yinpujiedu tablets by HPLC.Methods The HPLC method was used to determine the content of aesculetin in yinpujiedu tablets,and the HPLC conditions were with the chromatographic column Inert S ustain C18(4.6 mm×250 mm),methane alachol:0.1%phosphoric acid water(85∶15)as mobile phase elution,detection wavelength of 334 nm,column tenperature 30℃,the flow rate of 1 ml/min.Results The aesculetin was found to have a good linear relationship between 0.256μg and 1.536μg,RSD=0.688%,linear regression equation was Y=259.81X-193.69,the linear range of 0.256μg^1.536μg,r=0.999 9.Conclusion The method has high precision and accuracy.However,the results of the content determination of aesculetin in yinpujiedu tablets were relatively low.As a quality standard of yinpu jiedu tablets,the content determination of aesculetin has yet to be investigated.
作者
吴芝园
徐雪莲
王青萍
WU Zhi-yuan;XU Xue-lian;WANG Qing-ping(Suzhou Vocational Health College,Jiangsu Suzhou 215009,China.)
出处
《中国处方药》
2019年第11期20-21,共2页
Journal of China Prescription Drug
基金
苏州卫生职业技术学院院级课题(Szwzy201805)
关键词
高效液相色谱法
银蒲解毒片
秦皮乙素
含量测定
High performance liquid chromatography
Yinpu Jirdu Tablets
Aesculetin
Content determination
