摘要
建立了HPLC同时检测竹叶中4种黄酮碳苷(异荭草苷、荭草苷、牡荆苷和异牡荆苷)的方法,并测定了这4种常见的黄酮碳苷在24种刚竹属竹叶的含量,以及早园竹4个季度中黄酮碳苷的含量。采用InertsilODS-3色谱柱(250mm×4.6mm,5μm),以乙腈/0.5%磷酸水溶液(体积比15∶85)为流动相进行等度洗脱,流速为1.0mL/min,柱温为30℃,检测波长340nm。实验结果表明:4种黄酮碳苷的质量浓度在1~300mg/L范围内与峰面积呈良好的线性关系,相关系数均在99%以上,加标回收率(n=3)分别为97.14%、100.53%、93.60%和95.66%,RSD值分别为1.11%、1.45%、1.96%和1.43%;24种刚竹属竹叶中黄酮碳苷含量差异显著,4种黄酮碳苷质量分数的总和在286.14~3838.79mg/kg之间,毛金竹最高,为3838.79mg/kg;早园竹中4种黄酮碳苷的含量随时间变化差异明显,质量分数的总和在1371.15~4367.01mg/kg之间,其中1月份采摘时总质量分数最高,为4367.01mg/kg。HPLC法准确度高,适用于竹叶黄酮碳苷的测定。
An HPLC method was developed and validated for simultaneous determination of isoorientin, orientin, vitexin, and isovitexin, and the four common flavone C-glycosides in bamboo leaves of 24 species of Phyllostachys and their contents in P. propinqua in the four seasons. The HPLC separation was performed on an Inertsil ODS-3 (250 mm × 4. 6 mm, 5 μm) column with the mobile phase of acetonitrile (B) and 0. 5% phosphoric acid in water (C) with equal elution at a flow rate of 1 mL/ min. The column temperature was 30 ℃, and the detected wavelength was 340 nm. The results showed that the linear ranges of the concentration of isoorientin, orientin, vitexin and isovitexin were 1 -300 mg/ L, respectively, and the concentrations of the four compounds showed a good linear relationship with their peak areas, and the correlation coefficients all > 99%;the average recoveries for the samples were 97. 14%, 100. 53%, 93. 60% and 95. 66%, respectively, and the relative standard deviations were 1. 11%, 1. 45%, 1. 96% and 1. 43%, respectively. The results showed that the content of flavone C-glycosides had big differences among bamboo leaves of 24 species of Phyllostachys. The total content of the four flavone C-glycosides were in the range of 286. 14 -3 838. 79 mg/ kg. The maximum content was found in P. nigra, and its content was 3 838. 79 mg/ kg. The total concentrations of four flavone C-glycosides in P. propinqua were in the range of 1 371. 15 - 4 367. 01 mg/ kg in the 4 months, and the maximum content was found in January, and its content was 4 367. 01 mg/ kg. HPLC method had good reproducibility and high accuracy. This provides a basis for quality control of flavone C-glycosides of bamboo leaves.
作者
陈丹丹
郭雪峰
赵蕾
邵思越
CHEN Dandan;GUO Xuefeng;ZHAO Lei;SHAO Siyue(SFA Key Laboratory of Bamboo and Rattan Science and Technology,InternationalCentre for Bamboo and Rattan, Beijing 100102, China)
出处
《林产化学与工业》
EI
CAS
CSCD
北大核心
2019年第5期108-114,共7页
Chemistry and Industry of Forest Products
基金
国家自然科学基金面上项目资助(31570345)
关键词
HPLC
刚竹属竹叶
黄酮碳苷
定量分析
HPLC
bamboo leaves of Phyllostachys
flavone C-glycosides
quantitative analysis
