摘要
采用高效薄层色谱法(HPTLC)检测箣竹属、牡竹属及刚竹属11个竹种竹叶中牡荆苷、异牡荆苷、荭草苷、异荭草苷和苜蓿素。采用自动多级展开法,5种黄酮类化合物分离效果良好,其R F值分别为0.25,0.32,0.38,0.47和0.88,回收率在79.01%～ 106.85%之间。3属11种竹叶中黄酮类化合物种类和含量具有差异,紫竹中5种黄酮含量总和最高,为0.132%;麻竹中5种黄酮含量总和最低,为0.015%。
Isoorientin,orientin,isovitexin,vitexin and tricin in the leaves of three genera 11 bamboos been compared by a simple high-performance thin-layer chromatographic（ HPTLC） method. The multistage development was performed in Automated Multiple Development（ AMD2） and solvents with different ratios were used as mobile phase. The developed plates were scanned by TLC Scanner3. The components were separated well and R F of the five flavonoids 0. 25,0. 32,0. 38,0. 47 and 0. 88 respectively. The method was validated for precision,accuracy,selectivity and repeatability,and expressed as coefficient of variation（ CV [% ]）. The kinds of flavonoids and the concentrations in the eleven species bamboo of three different genera were significantly different. Phyllostachys nigra the maximum total flavonoids of 0. 132%,and the minimum of 0. 015%. The results would be useful in extracting natural bamboo-leaf flavonoids.
出处
《林业科学》
EI
CAS
CSCD
北大核心
2013年第10期127-134,共8页
Scientia Silvae Sinicae
基金
This research was supported by the State Forestry Administration,P.R.China 948 Project(2009-4-61)
the National Science and Technology Infrastructure Program(2012BAD23B03)
the Basic Science Research Fund Program of the International Centre for Bamboo and Rattan(ICBR)(12618-6)
