摘要
目的研究分析不同状态的标本对荧光定量PCR检测HBV DNA的影响。方法采用实时荧光定量PCR方法分别检测不同程度模拟溶血(高溶血组、中溶血组、低溶血组)和模拟脂血(高脂组、中脂组和低脂组)标本的HBV DNA浓度。选择HBV DNA阳性患者,每人分别抽取EDTA-K2和肝素抗凝血各1份,立即分离血浆作HBV DNA定量检测。对HBV DNA载量进行配对t检验,并进行统计分析。结果不同溶血程度对HBV DNA载量没有影响,差异无统计学意义(P>0.05),但当血红蛋白浓度达到113g/L时,HBV DNA载量下降明显;高脂组、中脂组和低脂组HBV DNA载量差异无统计学意义(P>0.05),但甘油三酯浓度为6.21mmol/L时,HBV DNA结果明显偏低;EDTA-K2抗凝血浆组与肝素抗凝血浆组的检测结果差异有统计学意义(P<0.05)。结论血红蛋白浓度低于113g/L的溶血标本、甘油三酯浓度低于6.21mmol/L的脂血标本对HBV DNA检测结果无明显影响。EDTA-K2抗凝血适用于HBV DNA检测,而肝素钠抗凝血则不合适。
Objective To study the influence of different sample status on the detection of HBV一DNA by fluorescence quantitative PCR. Methods Real-time quantitative PCR was used to detect HBV DNA concentrations in different degrees of simulated hemolysis ( high hemolysis group, middle hemolysis group, low hemolysis group) and simulated lipemia ( high fat group, middle fat group and low fat group). HBV DNA-positive patients were selected, and each person was given EDTA-K2 and heparin anticoagulant, and the plasma was immediately separated for HBV DNA quantitative detection. Paired t-test was performed on HBV DNA load and statistical analysis was performed. Results Different hemolysis degrees and TG concentrations had no effect on HBV-DNA load, and there was no statistical significance (P >0.05). But, when the hemoglobin concentration reached 113 g/L, the HBV DNA load decreased significantly;the differences of HBV DNA load in the high-fat group, the middle-fat group and the low-fat group were not statistically significant (P >0.05). When the TG concentration was 6.21 mmol/L, the HBV-DNA load significantly decreased. The difference between EDTA-K, anticoagulant plasma groups and heparin anticoagulant plasma groups was statistically significant (P <0.05). Conclusion Hemolysis specimens with a hemoglobin concentration of less than 113 g/L and lipoprotein specimens with a triglyceride concentration of less than 6.21 mmol/L had no significant effeet on the detection results of HBV DNA. EDTA-K, anticoagulation is indicated for HBV DNA testing, while heparin sodium anticoagulation is not suitable.
作者
赵云
刘兴祥
徐云芳
王莉娟
堵妍
崔婷
ZHAO Yun;LIU Xing-xiang;XU Yun-fang;WANG Li-juan;DU Yan;CUI Ting(Institute of Hepatology,the Fourth People's Hospital of Huai'an,Huai'an,Jiangsu 223002,China)
出处
《中国卫生检验杂志》
CAS
2019年第18期2254-2256,2259,共4页
Chinese Journal of Health Laboratory Technology
关键词
溶血
高脂血
抗凝剂
荧光定量聚合酶链反应
Hemolysis
Lipoidemia
Anticoagulaion
Fluorescence quantitative PCR
