摘要
目的研究不同程度溶血对荧光定量PCR检测不同含量HBVDNA的影响。方法利用荧光定量PCR方法研究溶血对其检测不同含量HBVDNA的影响,并利用SPSS17.0对结果进行统计学分析。结果极重度溶血组(12.0g/LHb)分别与高、中、低3个拷贝程度(106～107IU/mL、104～105IU/mL、102～103IU/mLHBVDNA)的对照组比较,其结果差异具统计学上意义(P<0.05)。极重度溶血时HBVDNA定量结果偏低,而轻度溶血组(1.5g/LHb)、中度溶血组(3.0g/LHb)及重度溶血组(6.0g/LHb)的HBVDNA定量测定结果与对照组比较,它们之间的差异均无统计学上意义(P>0.05)。结论低拷贝到高拷贝组HBVDNA的荧光定量检测均受极重度溶血的影响。对于此类样本,临床必须重新留样检测。而重度溶血、中度溶血及轻度溶血则对各拷贝组的HBVDNA荧光定量检测没有多大影响,无须重新留样。
Objective To investigate the influence of hemolysis on the detection of HBV DNA with different concentrations by fluorescence quantitative PCR（FQ-PCR）. Methods The effect of hemolysis on HBV DNA detection was studied by FQ-PCR, and a statistical analysis for the result was done by SPSS17.0. Results HBV DNA concentration decreased when the concentration of hemoglobin was up to 12.0g/L（P〈0.05）. There was no statistical significant difference between other experimental groups（1.5 g/L Hb, 3.0 g/L Hb, 6.0 g/ L Hb） and control groups in quantitative values of HBV DNA （10^6-10^7 IU/mL, 10^4-10^5 IU/mL, 10^2-10^3 IU/ mL HBV DNA） （P〉0.05）. Conclusion Severe hemolysis（12.0 g/L Hb, not 〈6.0g/L Hb） inhibits the FQ-PCR amplification of HBV DNA from low to high copy groups（10^02 - 10^7 IU/mL HBV DNA） while others do not, thus it is necessary to recollect the sample with severe hemolysis.
出处
《分子诊断与治疗杂志》
2012年第3期189-192,共4页
Journal of Molecular Diagnostics and Therapy
