摘要
目的 探讨荧光定量PCR检测HBV-DNA的临床应用价值。方法 对184例临床血清标本采用荧光定量PCR法进行HBV-DNA检测,同时采用酶联免疫吸附试验(ELISA)法进行乙肝免疫学比对检测。结果 经荧光定量PCR检测,42例HBsAg(+)、HBeAg(+)、HBcAb(+)的标本,HBV-DNA阳性率为100.0%(42/42);45例HBsAg(+)、HBeAb(+)、HBcAb(+)的标本,HBV-DNA阳性率为44.4%(20/45);28例HBsAb(+)、HBeAb(+)、HBcAb(+)的标本,HBV-DNA阳性率为14.3%(4/28);51例五项检测均为阴性的标本,HBV-DNA阳性率为2.0%(1/51)。结论 荧光定量PCR能够检测HBV的感染和复制情况,对准确报告HBV的感染,指导其选择治疗方案和观察疗效具有重要的临床意义。
Objective To explore the value in application of Fluorescence quantitative PCR in detection of HBV-DNA in physical examination.Methods Contents of HBV-DNA and the HBV serum markers were determined by Fluocrescence quantitative PCR technique and ELISA respectively in 184 serum samples.Results 42 HBsAg(+),HBeAg(+),HBcAb(+)samples were all positive result 100.0%(42/42);In 45 HBsAg(+),HBeAb(+),HBcAb(+)samples,the positive rate is 44.4%(20/45);In 28 HBsAb(+),HBeAb(+),HBcAb(+)samples,the positive rate is 14.3%(4/28);51 normal samples with a positive of 2.0%(1/51).Conclusion Fluorescence quantitative PCR was accurate for detection of HBV and can be used for monitoring the actual state of HBV infection and replication.
作者
张朝典
ZHANG Chaodian(Department of Clinical Laboratory,Hospital Directly Affiliated of Hubei,Wuhan,Hubei 430071,China)
出处
《检验医学与临床》
CAS
2018年第A01期96-98,共3页
Laboratory Medicine and Clinic
