摘要
目的 :筛选细辛抗人乳头瘤病毒 (HPV)的有效部位。方法 :用系统溶媒 (石油醚、乙醚、乙酸乙酯、正丁醇、乙醇、蒸馏水 )对细辛依次进行提取 ,应用荧光定量聚合酶链反应技术 (FQ PCR)对各提取物进行体外抗病毒药效学试验 ,检测离体尖锐湿疣皮损中HPV DNA的扩增情况。结果 :HPV DNA经细辛水提物作用后 ,其PCR扩增检测为阴性 ,最低有效浓度为 0 4 g/ml,而细辛其它提取部位PCR扩增检测为阳性。结论
Objective:To screen anti HPV effective fraction from Asarum heterotropoides Methods: Asarum heterotropoides was extracted with systematic solvents (petroleum benzin,ether,ethyl acetate,n butanol,ethanol and distilled water) accordingly The Polymerase Chain Reaction (FQ PCR) was applied in the vitro pharmacodynamics for the extracts from Asarum heterotropoides The amlification of HPV DNA in the lesions of the isolated Condyloma acuminatum Results:After the water extracts of Asarum heterotropoides was used for HPV DNA,the PCR amplification showed negative,with the minimum effective concentration 0 4g/ml But the other solvents extracts from Asarum heterotropoides appeared positive Conclusion:The water extracts from Asarum heterotropoides are effective on anti Human papillomavirus
出处
《中药材》
CAS
CSCD
北大核心
2004年第9期665-667,共3页
Journal of Chinese Medicinal Materials
基金
20 0 0年度广东省中医药管理局资助课题 (10 0 0 41)
