摘要
盐胁迫严重影响桉树的存活和生长。以广林9号桉3月龄幼苗为材料,用不同浓度NaCl溶液(0、30、60、90、120、150mmol/L)连续灌根处理15d后,检测叶组织渗透势、叶绿素含量以及Rbcl、Accd、Ndhf、Matk4个叶绿体基因的转录情况,分析盐胁迫条件下桉树叶绿体基因的表达变化。随着NaCl浓度的增大:组织液渗透势呈上升趋势,叶绿素质量分数呈下降趋势,当NaCl浓度为150mmol/L时组织液渗透势达最高值,2136.4kPa,叶绿素质量分数最低,为2.80mg/g;Rbcl、Ndhf、Matk基因表达水平呈降低趋势,Accd基因的表达水平随盐浓度升高而呈上升趋势。90mmol/LNaCl使Accd、Ndhf、Matk基因表达应激性升高。150mmol/LNaCl严重抑制Rbcl、Ndhf、Matk基因的表达,却使Accd基因表达显著增强。结果表明:90mmol/LNaCl浓度是桉树短期耐受盐胁迫的临界点,叶绿体中不同类型基因响应盐胁迫的应答不同。
Salt stress seriously affects the survival and growth of eucalyptus. The three-month-old eucalyptus saplings (Guanglin 9) were irrigated root with NaCl solution (0, 30, 60, 90, 120, and 150 mmol/L) for 15 d. Then, osmotic potential, chlorophyll content and the transcriptional levels of four chloroplast genes ( Rbcl, Accd, Ndhf and Matk ) in leaf tissue were evaluated. With the increase of NaCl concentration, the osmotic potential of tissue liquid showed an upward trend and the content of chlorophyll showed a decreasing trend. At 150 mmol/L NaCl, the osmotic potential was 2 136.4 kPa and the chlorophyll content was 2.80 mg/g. With the increase of salt concentration, the expression of Rbcl, Ndhf and Matk genes was decreased, however, the expression of Accd gene was increased. The transcriptional level of Rbcl, Ndhf and Matk increased significantly at 90 mmol/L NaCl. And it was suppressed markedly at 150 mmol/L NaCl. To our astonished, the transcriptional level of Accd was the highest at 150 mmol/L NaCl. The results implied that 90 mmol/L NaCl was the critical concentration for Eucalyptus to tolerate short-term salt stress. And the genes in chloroplasts show different response to salt stress.
作者
黄永健
蔡冠龙
潘东阳
黄真池
Huang Yongjian;Cai Guanlong;Pan Dongyang;Huang Zhenchi(Lingnan Normal University, Zhanjiang 524048, P. China;Lingnan Normal University)
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2019年第10期12-15,共4页
Journal of Northeast Forestry University
基金
国家自然科学基金项目(31570660)
广东省自然科学基金项目(2016A030313668)
大学生创新创业项目
关键词
盐胁迫
桉树
叶绿体
基因表达
Salt stress
Eucalyptus
Chloroplast
Gene expression
