摘要
本试验旨在研究补喂复合益生菌制剂对幼犬生长性能、肠道动力及肠道屏障功能的影响。选取40只6周龄左右的贵宾犬,随机分为5组,每组2个重复,每个重复4只犬。其中,试验A组幼犬饲喂基础饲粮+0.2 g/(kg BW·d)复合益生菌制剂,试验B组幼犬饲喂基础饲粮+0.6 g/(kg BW·d)复合益生菌制剂,试验C组幼犬饲喂基础饲粮+1.0 g/(kg BW·d)复合益生菌制剂,阴性对照组幼犬饲喂基础饲粮,对比对照组幼犬饲喂基础饲粮+1.0 g/(kg BW·d)宠儿香康源益生菌,进行为期30 d的饲养试验。试验结束后取样,用酶联免疫吸附测定(ELISA)法检测血清胃肠激素含量和肠道屏障功能指标,免疫比浊法检测血清炎性细胞因子含量,实时荧光定量PCR(RT-qPCR)法检测空肠黏膜中相关基因mRNA的相对表达量。结果显示:1)随着复合益生菌制剂补喂剂量的增加,终末体重和平均日增重增加,腹泻率和便秘率降低,其中试验C组平均日增重显著高于其他各组(P<0.05),腹泻率和便秘率显著低于阴性对照组(P<0.05)。2)随着复合益生菌制剂补喂剂量的增加,血清中胃动素(M TL)、白细胞介素-10(IL-10)含量呈升高趋势,血管活性肠肽(VIP)、D-乳酸、内毒素(LPS)、肿瘤坏死因子-α(TNF-α)含量与二胺氧化酶(DAO)活性呈降低趋势,其中试验C组与阴性对照比较差异显著(P<0.05),与对比对照组相比差异不显著(P>0.05)。3)随着复合益生菌制剂补喂剂量的增加,回肠黏膜闭合蛋白(occludin)、闭锁小带蛋白-1(ZO-1) mRNA的相对表达量呈上调趋势,肌球蛋白轻链激酶(MLCK) mRNA的相对表达量呈下调趋势,其中试验C组与阴性对照组差异显著(P<0.05),与对比对照组差异不显著(P>0.05),且ZO-1 mRNA的相对表达量显著高于试验A组和试验B组(P<0.05)。以上结果表明,补喂复合益生菌制剂可增强幼犬胃肠动力和肠道屏障功能,缓解炎症应激,改善生长性能,以每天补喂1.0 g/kg BW效果最佳。
The purpose of this experiment was to study the effects of supplementary feeding complex-probioticpreparation on growth performance,intestinal motility and intestinal barrier function of puppies. Forty poodles at the age about 6 weeks were randomly divided into five groups with 2 replicates in each group and 4 poodles in each replicate. Among them,poodles in test group A were fed a basal diet+ 0.2 g/(kg BW ·d) complexprobiotic-preparation;poodles in test group B were fed the basal diet+0.6 g/(kg BW ·d) complex-probioticpreparation;poodles in test group C were fed the basal diet+ 1. 0 g/(kg BW ·d) complex-probiotic-preparation;poodles in negative control group were fed the basal diet;and poodles in contrast control group were fed the basal diet+1.0 g/(kg BW ·d) G-pet probiotic powder. The experiment lasted for 30 days. After experiment to sampling,the contents of gastrointestinal hormones and intestinal barrier function indicators in serum were detected by enzyme-linked immunosorbent assay(ELISA) method,serum inflammatory factor contents were detected by immunoturbidimetry,and mRNA relative expression levels of related genes in jejunum mucosa were detected by quantitative real-time fluorescence PCR(RT-qPCR). The results showed as follows: 1)with the increase of feeding dosage of complex-probiotic-preparation,the final weight and average daily gain were increased,and the diarrhea rate and constipation rate were decreased. The average daily gain of test group C was significantly higher than that of other groups(P<0.05),and the diarrhea rate and constipation rate were significantly lower than those of negative control group(P<0.05). 2) With the increase of feeding dosage of complex-probiotic-preparation,the contents of motilin(MTL) and interleukin-10(IL-10) in serum showed an increase trend,while the contents of asoactive intestinal peptide(VIP),D-lactic acid,lipopolysaccharide(LPS) and tumor necrosis factor-α(TNF-α) and the activity of diamine oxidase(DAO) in serum showed a decrease trend. Significant differen
作者
陆江
朱道仙
卢鹏飞
张东豪
刘静
傅宏庆
LU Jiang;ZHU Daoxian;LU Pengfei;ZHANG Donghao;LIU Jing;FU Hongqing(Department of Pet Science and Technology, Jiangsu Agri-Animal Husbandry Vocational College, Taizhou 225300, China;Department of Animal Medicine, Jiangsu Agri-Animal Husbandry Vocational College, Taizhou 225300, China;Abram(Jiangsu) Animal Heath Co., Ltd., Taizhou 225300, China)
出处
《动物营养学报》
CAS
CSCD
北大核心
2019年第9期4242-4250,共9页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
江苏省大学生创新创业训练计划项目(201812806015Y)
江苏农牧科技职业学院横向课题配套项目(NSFPT201845,NSFPT201842)
关键词
复合益生菌制剂
幼犬
生长性能
肠道动力
肠道屏障功能
complex-probiotic-preparation
puppy
growth performance
intestinal motility
intestinal barrier function
