摘要
目的了解吉林省2017年肠道病毒71型和柯萨奇病毒A组16型(CVA16)流行特征、种系进化及基因分型。方法对吉林省2017年分离获得的EV71和CVA16阳性毒株的VP1编码区核苷酸进行扩增及序列测定,而后使用Mega 6.0和Bioedit 7.01软件进行序列分析。结果 8株EV71流行株均为C4a基因亚型,流行株间核苷酸和氨基酸同源性分别为92.2%~100%和98.3%~100%;9株CVA16流行株均为B1b基因亚型,流行株间核苷酸和氨基酸同源性分别为94.2%~100%和99.3%~100%。结论 EV71的C4a基因亚型和CVA16的B1b基因亚型是引起吉林省2017年手足口病流行的主要基因亚型;相对于EV71流行株的变异而言,CVA16流行株变异较少。
Objective To understand the epidemic characteristics,phylogenetic and genotyping of enterovirus group A type 71 and the coxsackievirus group A type 16 in Jilin Province,2017. Methods To amplificate and analyze the sequence of VP1 of EV71 and CVA16 positive strains isolated from Jilin Province in 2017,and then use Mega 6. 0 and Bioedit software 7. 01 for analysis. Results Eight strains of EV71 were C4a subtype,and its nucleotide and amino acid homology were respectively within 92. 2%-100% and 98. 3%-100%;nine strains of CVA16 were B1b subtype,and its nucleotide and amino acid homology were respectively within 94. 2%-100% and 99. 3%-100%. Conclusion C4a subtype of EV71 and B1b subtype of CVA16 are the major gene subtypes in Jilin Province in 2017. Compared to the variation of EV71 endemic plant,CVA16 endemic plant has less variation.
作者
魏雷雷
吴东林
杨尧
王岙
王爽
单元春
WEI Lei-lei;WU Dong-lin;YANG Yao;WANG Ao;WANG Shuang;SHAN Yuan-chun(Jilin Provincial Center for Disease Control and Prevention,Changchun,Jilin 130062,China)
出处
《中国卫生检验杂志》
CAS
2019年第17期2078-2079,2083,共3页
Chinese Journal of Health Laboratory Technology
基金
国家科学技术重大专项艾滋病和病毒性肝炎等传染病防治(2017ZX10103007-003)
吉林省手足口病病原谱分析(2017J028)
关键词
肠道病毒71型
柯萨奇病毒A组16型
手足口病
VP1编码区
Enterovirus group A type 71
Coxsackievirus group A type 16
Hand-foot-mouth disease
VP1 coding region
