摘要
目的分析吉林省2011年手足口病(hand,foot and mouth disease,HFMD)死亡病例标本中分离获得的肠道病毒71型(enterovirus 71,EV71)毒株全基因组基因特征。方法采用分段RT-PCR法对2011年吉林省HFMD死亡病例EV71分离株JL2011-56全基因组进行扩增和测序。利用Clustal X2和MEGA5软件构建分离株JL2011-56与Gen Bank中27株参考株的全基因组序列和VP1区全基因组序列的进化树,分析Jl2011-56株的流行病学特征;同时与Gen Bank中12株C4a基因亚型参考株进行全基因组核苷酸和氨基酸序列的同源性分析。结果 JL2011-56分离株属于C4a亚型,核苷酸序列和氨基酸序列与山东分离株SDLY107(JX244186.1)同源性最高,分别为99.2%和99.5%,与EV71原型株Br Cr的同源性分别为79.9%和95.7%。结论 JL2011-56分离株与山东株亲缘关系较近,可能存在共同的与中枢神经毒力相关的氨基酸突变位点。
Objective To analyze the genetic characteristics of whole genome of enterovirus 71（EV71） isolated from patients who died from hand, foot and mouth disease（HFMD）in Jilin Province in 2011. Methods The whole genome of EV71 JL2011-56 strain isolated from the patients who died from HFMD in Jilin Province in 2011 was amplified and sequenced by segmented RT-PCR. Phylogenetic tree was constructed based on the whole genome sequences and genome sequences of VP regions of JL2011-56 strain and 27 reference strains in Gen Bank by using Clustal X2 and MEGA5 software to analyze the epidemiological characteristics of the strains. Meanwhile, the homologies of nucleotide and amino acid sequences of JL2011-56 strain to 12 reference strains of subgenotype C4 a were analyzed. Results JL2011-56 strain belonged to C4 a genotype, of which the homologies of nucleotide and amino acid sequences were 99. 2% and 99. 5% to those of SDLY107 strain isolated in Shandong, and 79. 9% and 95. 7% to those of prototype strain Br Cr, respectively.Conclusion JL2011-56 strain was closely related to the strains isolated in Shandong, in which the common amino acid mutation sites associated with the central nerve virulence possibly existed.
出处
《中国生物制品学杂志》
CAS
CSCD
2017年第11期1146-1149,共4页
Chinese Journal of Biologicals
基金
吉林省人社厅2010年度留学回国人员科技创新创业项目(2010-27)
国家科学技术艾滋病和病毒性肝炎等重大传染病防治专项传染病监测技术平台项目(2012ZX10004209-003)
关键词
手足口病
肠道病毒71型
全基因组
Hand, foot and mouth disease （HFMD）
Enterovirus 71 （EV71）
Whole genome
