摘要
SVP(SHORT VEGETATIVE PHASE)是MADS-box家族一员,它能够整合多条开花途径的开花信号,调节植物由营养生长向生殖生长的转变。为了解梅花(Prunus mume Sieb.etZucc.)成花转变过程的分子机理,该研究采用RT-PCR方法从梅花‘长蕊绿萼’中克隆到2个SVP的同源基因,分别命名为PmSVP1和PmSVP2,并采用荧光定量PCR对2个基因的表达模式进行分析。序列分析表明,PmSVP1和PmSVP2的编码区长度分别为687和672bp,分别编码228和223氨基酸。系统进化结果显示,PmSVP1与拟南芥AtSVP以及一些木本植物SVP同源基因聚为一组,PmSVP2与马铃薯、乳浆大戟等草本植物中的SVP同源基因聚为一组。实时荧光定量分析表明,在成年梅花中,2个PmSVP基因主要在茎、叶和叶芽等营养器官中表达,且都在叶中表达量最高。在1月龄幼苗中,PmSVP1基因在根、茎、叶中都有表达,PmSVP2基因则没有任何表达;在梅花花芽分化过程中,PmSVP1和PmSVP2基因的表达量均呈现下调表达的趋势。研究推测,PmSVP1和PmSVP2基因可能参与调控梅花从营养生长向生殖生长的转变。
The MADS-box gene SVP ( SHORT VEGETATIVE PHASE ) integrates multiple flowering signals to regulate the transition from vegetative to reproductive development in plants . To better understand the molecular regulation of flower development in Prunus mume , we isolated two putative orthologs of SVP , PmSVP 1 and PmSVP 2. The expression patterns of PmSVP 1 and PmSVP 2 were determined by real-time RT-PCR. PmSVP 1 contains a 687 bp open reading frame (ORF) that encodes a peptide of 228 amino acid residues. PmSVP 2 contains a 672 bp ORF that encodes a peptide of 223 amino acid residues. Phylogenetic analysis suggested that PmSVP1 was closely related to Arabidopsis SVP, along with SVP-like members from some woody perennials, while PmSVP2 was closely related to EeDAM2 and StMADS11, which are SVP-like members from perennial herbs. To characterize the function of PmSVP 1 and PmSVP 2, we examined the spatial expression of PmSVP 1 and PmSVP 2 in different organs using qRT-PCR. Transcripts of PmSVP 1 and PmSVP 2 accumulated in vegetative organs, such as buds, stems and leaves. However, the expression level of PmSVP 2 was expressed only in adults and had low expression levels in vegetative organs, thus differing from PmSVP 1. Throughout the bud differentiation process, both PmSVP 1 and PmSVP 2 expression levels presented a downward trend. Once flower bud differentiation commenced, expression levels started to decrease, suggesting that these two genes may play an important role in the transition from the vegetative growth to the reproductive growth phase in P. mume.
作者
李玉舒
杨炜茹
程堂仁
王佳
张启翔
LI Yushu;YANG Weiru;CHENG Tangren;WANG Jia;ZHANG Qixiang(Beijing Key laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment, Landscape Architecture School, Beijing Forestry University, Beijing 100083, China;Beijing Vocational College of Agriculture, Beijing 102442, China)
出处
《西北植物学报》
CAS
CSCD
北大核心
2019年第7期1163-1171,共9页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家“863”计划(2013AA102607)
北京市共建项目专项
北京市职业院校教师素质提升计划
关键词
梅花
SVP
基因克隆
表达分析
Prunus mume
SVP gene cloning
expression analysis