摘要
以枇杷品系春花(具有腋芽成花特性)和品种‘火炬’(不具有腋芽成花特性)为试验材料,利用RNA-seq技术对枇杷腋芽进行转录组测序,以期筛选出调控枇杷腋芽成花的关键基因。结果表明:共鉴定到66 197条差异表达基因,其中上调表达基因主要集中在2月27日—4月8日;维恩图分析显示,不同取样点共同上调表达的基因有160个,共同下调表达的基因有198个。差异表达基因GO功能注释到3个大类(生物过程、细胞组分和分子功能)的48个功能组,KEGG通路显著性富集分析表明,差异基因主要被注释到20个途径上,富集于植物激素信号传导、淀粉和糖代谢、苯基丙烷代谢、植物-病原体互作反应和谷胱甘肽代谢等途径。经功能注释后,最终从腋芽转录组筛选到32个成花基因、1个ABA受体基因PYL4、1个ABA脱氢酶基因ABH4和大量功能未知的新基因,这些基因可能在枇杷腋芽成花途径中起重要作用。本研究有助于更好地了解枇杷腋芽成花过程中的基因转录水平,也可为进一步解析枇杷腋芽成花的分子遗传机制奠定基础。
In order to screen out the key genes regulating loquat axillary bud flowering,the transcriptome of loquat axillary buds was sequenced by RNA-seq technology,taking loquat line Chunhua( with axillary bud flowering characteristics) and variety ’Huoju ’(without axillary bud flowering characteristics) as experimental materials.The results showed that 66 197 differential expression genes were identified,of which the up-regulated genes were mainly concentrated from February 27 to April 8.Venn diagram analysis showed that 160 genes were up-regulated and 198 genes were down-regulated at different sampling points.Differential expression genes annotated by GO were involved in 48 functional groups of 3 major categories(biological process,cell component,and molecular function).The significant enrichment analysis of KEGG pathway showed that the differential expression genes were mainly annotated to 20 pathways,which were enriched in plant hormone signal transduction,starch and sugar metabolism,phenylpropane metabolism,plant pathogen interaction and glutathione metabolism.After functional annotation,32 flowering genes,1 ABA receptor gene PYL4,1 ABA dehydrogenase gene ABH4 and a large number of new genes with unknown functions were finally screened from the axillary bud transcriptome.These genes may play an important role in the flowering pathway of loquat axillary buds.This study will help to better understand the gene transcription level in the process of loquat axillary bud flowering,and also lay a foundation for further analyzing the molecular genetic mechanism of loquat axillary bud flowering.
作者
章加应
肖海涛
张学英
安海山
徐芳杰
周博强
ZHANG Jiaying;XIAO Haitao;ZHANG Xueying;AN Haishan;XU Fangjie;ZHOU Boqiang(Forest and Fruit Tree Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China;School of Ecological Technology and Engineering,Shanghai Institute of Technology,Shanghai 201418,China)
出处
《上海农业学报》
2022年第6期47-57,共11页
Acta Agriculturae Shanghai
基金
上海市科技兴农重点攻关项目[沪农科创字(2018)第1-7号]。
关键词
枇杷
腋芽成花
转录组分析
Loquat(Eriobotrya japonica Thunb.)
Axillary bud flowering
Transcriptome analysis
