摘要
采用超滤、Sephadex G-25、Sephadex G-15、反相高效液相色谱及质谱对榛仁分离蛋白降脂活性肽进行分离纯化及结构鉴定,并通过测定3T3-L1前脂肪细胞诱导分化过程中脂质积累、总胆固醇及甘油三酯水平,筛选出具有较高降脂活性的肽段。结果表明,经Sephadex G-15分离得到的C3组分的胰脂肪酶抑制、胆固醇胶束吸附及细胞降脂活性均显著高于其他组分。进一步经质谱解析筛选出的肽段Phe-Leu-Leu-Pro-His(FLLPH)与模型组相比,可抑制26.31%的总脂形成,降低32.67%胆固醇和23.87%甘油三酯水平。FLLPH具有较好的降脂活性,本研究可为榛仁降脂活性肽的开发提供理论参考。
A peptide with higher hypolipidemic activity was separated and purified from the alcalase hydrolysate of hazelnut protein isolate by ultrafiltration, Sephadex G-25 chromatography, Sephadex G-15 chromatography and reversed-phase highperformance liquid chromatography(RP-HPLC). The amino acid sequence of the peptide was identified by Q Exactive hybrid quadrupole-Orbitrap mass spectrometry. The levels of lipid accumulation, total cholesterol, and triglyceride in 3T3-L1 preadipocytes incubated with the peptide were also determined. The results showed that fraction C3 isolated by Sephadex G-15 chromatography had significantly higher inhibitory activity against pancreatic lipase, cholesterol micelle adsorption capacity,and cellular hypolipidemic activity than any other fractions. The peptide was identified as Phe-Leu-Leu-Pro-His(FLLPH),and it could inhibit 26.31% of total lipid accumulation in 3T3-L1 preadipocytes, and reduce cholesterol and triglyceride levels by 32.67% and 23.87%, respectively, compared to the model control. The present study provides a theoretical basis for the development of hypolipidemic peptides from hazelnut protein.
作者
周美含
郭勇
魏贞
赵兰
秦汉雄
王辑
闵伟红
ZHOU Meihan;GUO Yong;WEI Zhen;ZHAO Lan;QIN Hanxiong;WANG Ji;MIN Weihong(National Engineering Laboratory for Wheat and Corn Further Processing, College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2019年第16期124-129,共6页
Food Science
基金
国家高技术研究发展计划（863计划）项目（2013AA102206）
关键词
降脂活性肽
分离纯化
结构鉴定
3T3-L1前脂肪细胞
hypolipidemic peptides
isolation and purification
structural identification
3T3-L1 preadipocytes
