摘要
目的探讨N-乙酰半胱氨酸(NAC)对慢性阻塞性肺疾病(COPD)患者炎症水平的影响。方法COPD稳定期患者90例,随机分为对照组(44例)与治疗组(46例)。对照组给予基础治疗,治疗组在基础治疗上给予NAC600mg,po,bid,疗程6个月,比较两组患者治疗前后外周血血清中白细胞介素(IL)-6、IL-8水平及肺功能水平。同时提取COPD患者外周血单个核细胞(PBMC),观察香烟提取物(CSE)、CSE+NAC刺激后IL-6和IL-8的表达。结果治疗组治疗后血清IL-6、IL-8的水平较治疗前显著下降(P<0.05);而对照组治疗前后炎症因子的表达水平无明显变化(P>0.05)。PBMC所释放的炎症因子(IL-6、IL-8)水平在CSE刺激后显著升高(P<0.05),在加入NAC刺激后显著降低(P<0.05)。结论NAC可通过降低COPD患者的炎症反应而发挥治疗作用。
Objective To investigate the effect of N-acetylcysteine(NAC)on inflammatory markers in chronic obstructive pulmonary disease(COPD)patients by clinical randomized controlled trial and in vitro cell experiment.Methods Ninety COPD patients in a stable stage were randomly divided into treatment group(46 cases)and control group(44 cases).Patients of both groups received baseline therapy,and NAC(600 mg,po,bid)was used in the treatment group additionally for 6 mooths.The levels of interleukin 6(IL-6)and interleukin 8(IL-8),and pulmonary function of all patients were monitored and analyzed before and after treatment.Meanwhile,peripheral blood mononuclear cells(PBMC)of COPD patients were collected to study the expression levels of inflammatory cytokines IL-6 and IL-8 after cigarette smoke extract(CSE)or CSE plus NAC stimulation.Results The IL-6 and IL-8 levels in serum significantly decreased after treatment in the treatment group(P<0.05).However,the IL-6 and IL-8 levels in the control group did not significantly change after treatment(P>0.05).The IL-6 and IL-8 levels significantly increased after CSE stimulation in PBMC(P<0.05),and significantly decreased after NAC stimulation(P<0.05).Conclusion Antioxidant therapy may play a role in the treatment of COPD by reducing the inflammatory response.
作者
武小杰
陈菁
WU Xiaojie;CHEN Jing(Department of Respiratory,Wuhan Hospital of Traditional Chinese and Western Medicine,Wuhan 430022,China)
出处
《医药导报》
CAS
北大核心
2019年第9期1183-1187,共5页
Herald of Medicine
关键词
N-乙酰半胱氨酸
肺疾病
阻塞性
慢性
氧化应激
炎症反应
N-acetylcysteine
Pulmonary disease,obstructive,chronic
Oxidative stress
Inflammatory response
