摘要
目的探讨极性蛋白Par3(Par3蛋白)在慢性宫颈炎、宫颈上皮内瘤变Ⅱ~Ⅲ(CINⅡ~Ⅲ)、宫颈鳞状细胞癌(CSCC)以及转移淋巴结组织中的表达及临床意义。方法选择新疆医科大学第一附属医院妇科2016年1月~2017年12月收治的CINⅡ~Ⅲ、CSCC以及因良性疾病行子宫切除术患者宫颈组织标本存档石蜡包埋组织共93例,其中慢性宫颈炎14例、CINⅡ~Ⅲ36例、CSCC43例。另CSCC淋巴结转移灶组织标本7例。应用免疫组织化学染色法检测极性蛋白Par3在上述组织中的表达。结果Par3蛋白在慢性宫颈炎、CINⅡ~Ⅲ、CSCC和转移淋巴结组织中的阳性表达率分别为100.0%(14/14)、97.2%(35/36)、62.8%(27/43)、0,各组间差异均有统计学意义(P<0.05)。CSCC中高分化组Par3蛋白100%(7/7)表达,而中低分化组的表达下调为55.6%(20/36),差异有统计学意义(P<0.05);根据是否发生淋巴结转移分组(淋巴结转移组7例;未发生淋巴结转移组36例),Par3蛋白在淋巴结转移组宫颈组织中阳性表达率是28.6%(2/7),而非转移组为58.3%(21/36),差异有统计学意义(P<0.05)。Par3蛋白的低表达与组织学分化和发生淋巴结转移有关,差异有统计学意义(P<0.05)。结论极性蛋白Par3在慢性宫颈炎、CINⅡ~Ⅲ以及CSCC、淋巴结转移灶组织中的表达有差异,CSCC组织中表达下调,其异常低表达与CSCC发生发展有关,可以作为判断宫颈上皮内瘤变发展为CSCC预警指标以及判断CSCC恶性程度和预后的指标。
Objective To explore the polarity protein Par3(Par3 protein)in chronic cervicitis,cervical intraepithelial neoplasiaⅡ-Ⅲ(CINⅡ-Ⅲ),cervical squamous cell carcinoma(CSCC)and the expression differences in metastasis lymph node tissue and its clinical significance.Methods From January 2016 to December 2017,Xinjiang Medical University First Affiliated Hospital of CINⅡ-ⅢCSCC,as well as the line of hysterectomy for benign disease patients with cervical tissue samples archived paraffin embedding tissue,a total of 93 cases were seleeted from Department of Gynaecology.Among them 14 cases of chronic cervicitis,36 cases of CINⅡ-Ⅲ,and 43 cases of cervical squamous cell carcinoma.Another 7 cases of cervical squamous cell carcinoma lymph node metastasis tissue samples.The expression of polar protein Par3 in the above tissues was detected by immunohistochemical staining.Results The positive expression rate of Par3 protein in chronic cervicitis,CINⅡ-Ⅲ,CSCC and metastasis lymph node tissues were 100.0%(14/14),97.2%(35/36),62.8%(27/43),0 respectively,the differences between each group had statistical significance(P<0.05).The Par3 protein expression was 100%(7/7)in the high differentiation group of CSCC,while the expression was down-regulated to 55.6%(20/36)in the low and medium differentiation group,with statistically significant difference(P<0.05).They were grouped according to whether lymph node metastasis had occurred(7 cases in lymph node metastasis group 36 cases in the group without lymph node metastasis.The positive expression rate of Par3 protein in cervical tissues of the lymph node metastasis group was 28.6%(2/7),while that of the non-metastasis group was 58.3%(21/36),with statistically significant difference(P<0.05).The low expression of Par3 protein was correlated with histological differentiation and lymph node metastasis,and the difference was statistically significant(P<0.05).Conclusion Polarity protein Par3 in chronic cervicitis,CINⅡ-ⅢCSCC and are differentially expressed,the expression of CSCC
作者
郭春凤
赵骏达
马俊旗
GUO Chunfeng;ZHAO Junda;MA Junqi(Department of Gynecology,the First Affiliated Hospital of Xinjiang Medical University,Xinjiang Uygur Autonomous Region,Urumqi 830011,China)
出处
《中国医药导报》
CAS
2019年第21期93-96,F0004,共5页
China Medical Herald
基金
新疆维吾尔自治区自然科学基金面上项目(2017D01C304)
关键词
宫颈肿瘤
Par3蛋白
蛋白表达
宫颈上皮内瘤变
Uterine cervical neoplasms
Par3
Protein expression
Cervical intraepithelial neoplasia
