摘要
为了从分子水平上研究大豆籽粒不同发育时期的油脂合成与累积机理,对大豆开花20 d(DD20)、30 d(DD30)、40 d(DD40)、50 d(DD50)的籽粒进行了转录组测序。通过对转录组测序数据的分析,共得到原始数据461 566 988条,经过滤获得开花后20,30,40和50 d的clean reads,分别为107 548 920,111 670 776,109 339 672和108 884 270条。DD20/DD30、DD30/DD40、DD40/DD50比较组中的差异表达基因分别为4 759,6 245和13 763个,其中上调基因分别为1 801,2 941和5 695个。差异表达基因的KEGG pathway分析中,分别得到134,133和136条代谢通路,筛选到8个与油脂合成相关的差异表达基因,ACC、FATB、GPAT、DGAT1、G3PDH、KASI、SAD和FAD2。研究结果对深入研究大豆籽粒脂质合成的调控机理及大豆高油育种具有重要参考价值。
In order to study the mechanism of oil synthesis and accumulation in soybean seeds at different developmental stages at the molecular level, the soybean seeds of 20 d(DD20), 30 d(DD30), 40 d(DD40) and 50 d(DD50) were used as test materials in transcriptome sequencing. Through the analysis of transcriptome sequencing data, 461 566 988 raw reads were obtained. After filtration, the clean reads of DD20, DD30, DD40 and DD50 were obtained respectively, as 107 548 920, 111 670 776, 109 339 672 and 108 884 270. The total differential expression genes of DD20/DD30, DD30/DD40, DD40/DD50 were 4 759, 6 245 and 13 763 respectively, of which up-regulated genes were 1 801, 2 941 and 5 695 respectively. KEGG pathway analysis of differential expression genes obtained 134, 133 and 136 KEGG pathways, screened 8 differential expression genes related to lipid synthesis, as ACC, FATB, GPAT, DGAT1, G3 PDH, KASI, SAD and FAD2. The results provide an important reference for further study on the regulation mechanism of soybean seed lipid synthesis, as well as for the breeding of high oil content soybean varieties.
作者
陈锦玲
徐媛
陈玉梅
李璐璐
李惠敏
秦新民
CHEN Jin-ling;XU Yuan;CHEN Yu-mei;LI Lu-lu;LI Hui-min;QIN Xin-min(College of Life Science, Guangxi Normal University, Guilin 541004,China)
出处
《大豆科学》
CAS
CSCD
北大核心
2019年第4期533-541,共9页
Soybean Science
基金
广西研究生教育创新计划(XYCSZ2018056)
关键词
大豆
差异表达基因
油脂合成
转录组测序
Soybean
Differential expressed genes
Lipid synthesis
Transcriptome sequencing
