摘要
为探索甜菜对低温应答的分子机制,本研究对低温处理后甜菜叶片转录组变化进行测定。结果表明,低温处理24 h后共有3061个响应低温的显著地差异表达基因(DEGs)被鉴定。GO富集分析显示,这些DEGs主要在激素响应(GO:0009725)和激素介导的信号通路(GO:0009755)等12个方面被显著富集。KEGG分析同样显示植物激素信号转导(ko04075)是甜菜低温应答的主要方式。对参与这些通路的DEGs进行分类发现,参与生长素和脱落酸信号通路的DEGs占比最高,分别占41%和22%。说明生长素和脱落酸介导的信号转导可能在甜菜响应低温胁迫中起重要作用。在生长素信号通路中,分别有7个BvABP19a和17个BvSAUR受低温影响基因水平发生显著变化,从而调控甜菜生长发育。在脱落酸信号通路中,3个BvPYL、2个BvSAPK2和2个BvABF基因表达受低温影响显著,从而调控胁迫防御基因表达。本研究结果初步解析了甜菜在转录水平如何响应低温胁迫,为克隆甜菜抗低温关键基因提供了必要信息。
To explore the molecular mechanism of chilling response in sugar beet, the change of sugar beet leaf transcriptome after chilling was tested. The results showed that a total of 3061 differentially expressed genes(DEGs) responding to chilling were identified at 24 hours after chilling. GO enrichment analysis presented that these DEGs were significantly enriched in 12 GO terms, such as response to hormone(GO:0009725) and hormone-mediated signaling pathway(GO:0009755). KEGG analysis also showed that plant hormone signal transduction(ko04075) was also identified as the main pathway in response to chilling. The classification of DEGs involved in these pathways showed that the proportion of DEGs involved in auxin and abscisic acidmediated signaling pathways was the highest, accounting for 41% and 22%, respectively. It indicated that auxin and abscisic acid-mediated signaling pathways may play an important role in the response of sugar beet to chilling. In auxin-mediated signaling pathway, a total of 7 BvABP19 a and 17 BvSAUR were induced and inhibited by chilling, which probably regulated the growth and development of sugar beet. In abscisic acidmediated signaling pathway, the expression of 3 BvPYL, 2 BvSAPK2 and 2 BvABF were induced or inhibited by chilling, which were responsible for the expression of stress defense genes. The results of this study preliminarily reveal how sugar beet responds to low temperature stress at the transcriptional level and provide necessary information for cloning of cold-tolerance related key genes in sugar beet.
作者
龙佳丽
邹奕
邳植
吴则东
Long Jiali;Zou Yi;Pi Zhi;Wu Zedong(School of Life Sciences,Heilongjiang University,Harbin 150080;College of Advanced Agriculture and Ecological Environment,Heilongjiang University,Harbin 150080)
出处
《中国农学通报》
2021年第31期5-14,共10页
Chinese Agricultural Science Bulletin
基金
财政部和农业农村部国家现代农业产业技术体系
黑龙江省自然科学基金(LH2021C074)。
关键词
甜菜
低温胁迫
转录组
植物激素
生长素
脱落酸
sugar beet
low temperature stress
transcriptome
plant hormone
auxin
abscisic acid