摘要
目的:探讨急性心肌梗死患者血清caspase-12酶活性、CHOP蛋白、HSP47表达水平与冠状动脉(冠脉)病变范围的相关性。方法:纳入因急性心肌梗死入院的患者126例,按冠脉病变范围分为单支病变组(41例)、双支病变组(40例)和3支病变组(45例),留取患者发病24h内外周静脉血,采用酶联免疫吸附法(ELISA)测定血清caspase-12酶活性、CHOP蛋白、HSP47表达水平。采用方差分析比较3组间各指标的差异,采用Spearman检验分析caspase-12酶活性、CHOP蛋白、HSP47表达水平与冠脉病变范围的相关性。结果:①单支病变组、双支病变组和3支病变组年龄差异具有统计学意义(P<0.05);②caspase-12、CHOP及HSP47血清浓度在3组差异无统计学意义;③血管病变范围与HSP47呈正相关性(r=0.313,P=0.004),与CHOP、caspase-12血清浓度无相关性。结论:急性心肌梗死患者血清HSP47表达水平可能与冠脉病变范围有关。
Objective:To investigate the correlation between serum caspase-12 enzyme activity,CHOP protein,HSP47 expression levels,and the coronary artery lesions in acute myocardial infarction(AMI)patients.Method:A total of 126 patients with AMI were divided into single vessel disease group(n=41),double vessels disease group(n=40),and three vessels disease group(n=45)according to the scope of coronary artery disease.Peripheral venous blood was collected within 24 hours after the onset of AMI.Serum caspase-12 activity,CHOP protein and HSP47 expression levels were determined by enzyme-linked immunosorbent assay(ELISA).Variance analysis was used to compare differences of each index among three groups.Spearman test was used to analyze the correlation between caspase-12 enzyme activity,CHOP protein,HSP47 expression level and coronary artery lesion extent.Result:①There was a significant difference in age among three groups(P<0.05).②There was no significant difference in serum levels of caspase-12 enzyme activity,CHOP protein and HSP47 among three groups.③The range of vascular lesions was positively correlated with HSP47(r=0.313,P=0.004),but there was no correlation between the range of vascular lesions and serum concentration of CHOP and caspase-12.Conclusion:The level of serum HSP47 expression in patients with AMI might be related to the extent of coronary artery disease.
作者
田倪妮
田敏
杨俊
陈礼萍
赵仲文
马娟
苗文清
安丽媛
田青
TIAN Nini;TIAN Min;YANG Jun;CHEN Liping;ZHAO Zhongwen;MA Juan;MIAO Wenqing;AN LIyuan;TIAN Qing(Department of Cardiology,Kunming First People's Hospital, Kunming, 650011,China;Clinical Laboratory, Kunming First People's Hospital, Kunming, 650011,China;Kunming Medical University Graduate School)
出处
《临床心血管病杂志》
CAS
北大核心
2019年第7期631-633,共3页
Journal of Clinical Cardiology
基金
昆明市科技计划项目(No:2017-1-S-16167)
昆明卫生科技人才暨“十百千”工程(No:2018-SW后备-55
9)
云南省地方本科高校(部分)基础研究联合专项资金青年项目(No:2018)
