摘要
目的:为建立款冬花配方颗粒的质量标准提供参考。方法:采用薄层色谱法(TLC)对款冬花配方颗粒进行定性鉴别;采用高效液相色谱法测定款冬花配方颗粒中款冬酮的含量,色谱柱为Thermo ODS Hypersil C18,流动相为甲醇-水(85 ∶15,V/V),流速为1.0 mL/min,检测波长为220 nm,柱温为25 ℃,进样量为20 μL。结果:款冬酮TLC图斑点清晰、分离度良好,阴性对照对测定无干扰。款冬酮检测质量浓度的线性范围为1.39~27.75 μg/mL(r=0.999 9);定量限、检测限分别为0.153 87、0.051 42 μg/mL;精密度、稳定性、重复性试验的RSD均小于2%;加样回收率为97.12%~103.96%(RSD=2.60%,n=6)。结论:该方法操作简单、准确、重复性好,可用于款冬花配方颗粒的质量控制。
OBJECTIVE:To provide reference for the establishment of quality standard of Tussilago farfara formula granules. METHODS:TLC method was used for qualitative identification of tussilagone in T. farfara formula granules. The content of tussilagone in T. farfara formula granule was determined by HPLC. The determination was performed on a Thermo ODS Hypersil C18 column with the mobile phase consisted of methanol-water (85 ∶ 15,V/V) at the flow rate of 1.0 mL/min. The detection wavelength was set at 220 nm,the column temperature was 25 ℃. Sample size was 20 μL. RESULTS:TLC spots of tussilagone were clear and well-separated,without interference from negative control. The linear range of tussilagone was 1.39-27.75 μg/mL (r=0.999 9). The limits of quantification and detection were 0.153 87 and 0.051 42 μ g/mL,respectively. RSDs of precision, stability and reproducibility tests were lower than 2%. The recoveries were 97.12%-103.96%(RSD=2.60%,n=6). CONCLUSIONS: The method is simple,accurate and reproducible,and suitable for quality control of T. farfara formula granules.
作者
许洪波
蔡兴航
毛晶晶
杨康
许怀礼
XU Hongbo;CAI Xinghang;MAO Jingjing;YANG Kang;XU Huaili(Shaanxi Collaborative Innovation Center of TCM Resources Industrialization,Shaanxi University of TCM,Shaanxi Xianyang 712083,China;Yangling Charisma Bio-Pharmaceutical Co.,Ltd.,Xi’an 712100;Dept. of Pharmacy,Xi'an Traditional Chinese Medicine Hospital,Xi’an 710021,China)
出处
《中国药房》
CAS
北大核心
2019年第14期1898-1901,共4页
China Pharmacy
基金
国家自然科学基金资助项目(No.81603284)
陕西省教育厅科学研究计划项目(No.18JK0222)