摘要
目的探讨血浆微小RNA(microRNA,miRNA)-145和miRNA-221表达在非小细胞肺癌(non-small cell lung cancer,NSCLC)术后复发中的诊断价值。方法选取2016年4月~2018年4月期间在陕西中医药大学附属医院和陕西省核工业二一五医院胸外科行手术切除的55例原发性非小细胞肺癌患者的临床资料进行回顾性分析,依据术后是否复发分为复发组和未复发组.同时选取50例同期体检健康者作为对照组.采用实时聚合酶联反应(real-time polymerase chain reaction,RT-PCR)技术检测NSCLC患者血浆miRNA-145和miRNA-221的表达,采用电化学发光方法检测血清细胞角蛋白片断21-1(CYFRA21-1)和癌胚抗原(CEA)水平,比较分析以上标志物的变化与NSCLC患者术后复发的关系。结果在对照组、未复发组和复发组中,血浆miRNA-145和miRNA-221的表达分别为0.97±0.27,0.35±0.15,0.17±0.09和0.20±0.11,0.39±0.17,1.06±0.31。未复发组的miRNA-221表达显著高于对照组,而显著低于复发组;未复发组的miRNA-145表达显著低于对照组,而显著高于复发组;以上比较的差异均有统计学意义(F=118.73~123.72,均P<0.01)。在对照组、未复发组和复发组中,血清CYFRA21-1(ng/ml)和CEA(ng/ml)水平分别为3.02±1.17,9.05±3.43,47.68±16.72和2.31±2.03,7.37±3.29,29.37±12.49。复发组和未复发组的CYFRA21-1和CEA水平与对照组比较显著增高;复发组两肿瘤标志物水平与未复发组比较显著增高;以上比较差异均有统计学意义(F=87.46~96.83,均P<0.01)。在复发组中,血浆miRNA-145和miRNA-221表达具有负相关性(r^2=0.772,P<0.01)。miRNA-145表达分别与CYFRA21-1和CEA水平有负相关性(r^2=0.772~0.794,均P<0.01),而miRNA-221表达分别与CYFRA21-1和CEA水平有正相关性(r^2=0.663~0.687,均P<0.01)。血浆miRNA-221和miRNA-145表达在鳞癌中的异常率高于腺癌,在TNMⅢ期中的异常率高于Ⅰ-Ⅱ期(χ^2=0.012~0.039,均P<0.05);两标志物与患者年龄、肿瘤大小和吸烟无明显相关性(χ^2=0.301
Objective To investigate the diagnostic value of the expression of microRNA145 and microRNA221 in plasma for postoperative recurrence of non-small cell lung cancer(NSCLC).Methods The clinical data of 55 patients with primary NSCLC who underwent surgical resection at the Department of Thoracic Surgery in Affiliated Hospital of Traditional Chinese Medicine University of Shaanxi and 215 Hospital of Nuclear Industry of Shaanxi from April 2016 to April 2018 were retrospectively analyzed.According to whether the recurrence after surgery,they were divided into recurrent group and non-recurrent group.At the same time,50 cases of healthy people in the same period were selected as the control group.Real-time polymerase-linked quantitative(RT-PCR) technique was used to detect the expression of miRNA-145 and miRNA-221 in plasma of patients with NSCLC,serum levels of CYFRA21-1 and CEA were detected by electrochemiluminescence method.The relationship between the changes of the above markers and the recurrence of NSCLC patients was comparatively analyzed.Results In the control group,non-recurrence group and recurrence group,the expression of plasma microRNA-145 and microRNA-221 were 0.97±0.27,0.35±0.15,0.17±0.09 and 0.20±0.11,0.39±0.17,1.06±0.31,respectively.The expression of miRNA-221 in the non-recurrent group was significantly higher than that in the control group,but significantly lower than that in the recurrent group.The expression of miRNA-145 was significantly lower than that in the control group,and significantly higher than that in the recurrent group.The above differences were statistically significant(F=118.73~123.72,all P<0.01).The expression of serum CYFRA21-1(ng/ml) and CEA(ng/ml) in the control group,non-recurrence group and recurrence group were 3.02±1.17,9.05±3.43,47.68±16.72 and 2.31±2.03,7.37±3.29,29.37±12.49,respectively.Compared with the control group,the levels of CYFRA21-1 and CEA in the recurrent group and non-recurrence group were significantly increased.Compared with the non-recurrence gro
作者
黄刚
陈霏
肇玉博
张迪
HUANG Gang;CHEN Fei;ZHAO Yu-bo;ZHANG Di(Department of ClinicalLaboratory,Affiliated Hospital of Traditional Chinese Medicine University of Shaanxi,Shaanxi Xianyang 712000,China;215 Hospital of Nuclear Industry of Shaanxi,Shaanxi Xianyang 712000,China)
出处
《现代检验医学杂志》
CAS
2019年第4期40-44,共5页
Journal of Modern Laboratory Medicine
