摘要
目的观察Polo样激酶1（PLK1）在原发性肝癌细胞中的表达，探讨其与肝癌患者临床病理特点及预后的关系。方法采用免疫组织化学技术观察PLK1在40例原发性肝癌组织石蜡切片中的表达。结果癌栓、包膜侵犯、BCLC分期等因素和PLK1的阳性表达呈明显相关（P〈0．05）；PLK1阳性组和阴性组的术前甲胎蛋白（AFP）差异有统计学意义（P〈0．05）；秩和检验分析PLKl表达与患者肿瘤直径大小的关系发现PLK1阳性组和阴性组之间差异有统计学意义（P〈0．01）。生存分析显示，PLK1的表达和患者的生存期无明显相关；Kaplan—Meier法分析原发性肝癌的预后的单因素相关因子显示与包膜侵犯、TNM分期、癌栓及转移与生存期明显相关（P〈0．05），与性别、乙肝、肝硬化、肿瘤数目BCLC分期及临床分期无明显相关；多因素Cox回归分析显示包膜侵犯、癌栓及转移3项指标反映肝癌预后（P〈0．05）。结论PLK1的表达与肝癌的发展及其临床生物学行为密切相关。
Objective To investigate the expression of Polo-like kinase 1 （ PLK1 ） in patients with hepatoceilular cancer （HCC） , and find out its correlation with clinicopathological features and prognosis of HCC. Methods The expression of PLK1 in 40 patients with primary HCC was detected by using immuno- histochemistry （IHC） and analyzed with SPSS13.0 software. Results Significant positive correlations were revealed between PLK1 expression and tumor thrombosis, envelope evasion and BCLC staging （P 〈 0. 05 ）. Meanwhile, there were significant differences in preoperative alpha fetal protein （AFP） level and tumor diameter between PLK1 positive and negative groups （ P 〈 0. 05 ）. However, no significant correla- tion was observed between PLK1 expression and survival time of HCC. Factors, including envelope eva- sion, TNM staging, tumor thrombosis and metastasis, were significantly correlated with the survival time （ P 〈 0. 05 ） , while Cox regression analysis showed that envelope evasion, tumor thrombosis and metastasis were significantly correlated with HCC prognosis （P 〈 0. 05 ）. Conclusion PLK1 is closely correlated with the development and clinieobiological characteristics of HCC.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2011年第4期547-549,I0003,共4页
Chinese Journal of Experimental Surgery
关键词
癌
肝细胞
PLK1
免疫组织化学
预后
Carcinoma,hepatocellular
Polo-like kinase 1
Immunohistochemistry
Prognosis
