摘要
目的检测miR-129对鼻咽癌细胞系CNE2细胞生物学功能的影响。方法人工合成miR-129模拟物(mimics)和抑制物(inhibitor),转染至鼻咽癌CNE2细胞中,RT-qPCR检测细胞miR-129表达;CCK8法检测细胞增殖;Transwell小室侵袭实验检测细胞侵袭能力;流式细胞仪检测细胞凋亡能力。结果与对照组相比,转染miR-129mimics组的miR-129表达水平明显升高(P<0.05);转染miR-129inhibitor组的miR-129表达水平均明显降低(P<0.05);与对照组相比,转染miR-129mimics组CNE2细胞增殖能力、侵袭能力显著下降(P<0.05),凋亡显著增多(P<0.05);与对照组相比,转染miR-129inhibitor组CNE2细胞增殖能力、侵袭能力显著升高(P<0.05),凋亡显著下降(P<0.05)。结论miR-129抑制鼻咽癌细胞系CNE2的增殖和迁移能力,促进细胞凋亡。
Objective To examine the effect of mir-129 on the biological function of nasopharyngeal carcinoma cells.Methods miR-129 mimics and inhibitor were synthesized and transfected into nasopharyngeal carcinoma cells.Mir-129 expression was detected by RT-PCR.Cell proliferation was detected by CCK8.Cell invasiveness was detected by Transwell test.Cell apoptosis was detected by flow cytometry.Results Compared with the control group,the expression of mir-129 in the mir-129 mimics group was significantly increased(P<0.05),and the expression of mir-129 was significantly reduced in the mir-129 inhibitor group(P<0.05).Compared with the control group,the proliferation and invasion of CNE2 cells in the mir-129 mimics group were significantly decreased(P<0.05),and apoptosis was significantly increased(P<0.05).Correspondingly the proliferation and invasion significantly increased(P<0.05)and apoptosis of CNE2 cells in the mir-129 inhibitor group was significantly decreased(P<0.05).Conclusions miR-129 inhibites the proliferation and migration in nasopharyngeal carcinoma cells and promotes apoptosis.
作者
齐力
耿彬
宋丽华
QI Li;GENG Bin;SONG Li-hua(Department of Otolaryngology,the Affiliated Hospital of Inner Mongolia University,Tongliao 028000,China)
出处
《基础医学与临床》
CSCD
2019年第8期1178-1182,共5页
Basic and Clinical Medicine
