摘要
目的:探讨氯喹(CQ)对鼻咽癌细胞增殖和凋亡的影响。方法:采用MTT法检测不同浓度(0、2、4、8、16、32μmol/L)CQ对鼻咽癌细胞CNE-2Z的增殖抑制效应;集落克隆实验法检测不同浓度CQ对集落克隆形成的影响;溴化丙啶单染检测CQ诱导鼻咽癌细胞CNE-2Z凋亡;Western blot检测CQ(10μmol/L)处理鼻咽癌细胞CNE-2Z不同时间(0、6、16、24 h)内质网应激反应标志物葡萄糖调节蛋白(GRP-78)的表达。结果:不同浓度CQ均对鼻咽癌细胞CNE-2Z具有明显的增殖抑制作用,16μmol/L CQ作用于鼻咽癌细胞CNE-2Z 24、48、72 h细胞存活率分别为85.94%、75.34%和56.45%。同时,CQ具有抑制鼻咽癌细胞CNE-2Z的集落克隆形成的作用。并且,CQ呈剂量依赖性诱导鼻咽癌细胞CNE-2Z的凋亡。另外,CQ诱导鼻咽癌细胞上调GRP-78的表达。结论:CQ能抑制鼻咽癌细胞增殖,并通过引起过度的内质网应激反应机制诱导其凋亡。
Objective ：To investigate the effects of chloroquine （CQ） on the proliferation and apoptosis of nasopharyngeal carcinoma cells. Methods：The growth inhibition of CNE-2Z cells induced by different concentration CQ（ at 0,2,4,8,16,32 μmol/L of CQ） were detected by MTF assay. The effects of different concentration CQ on the colony formation were detected by colony cloning experiment. The apoptosis of CNE-2Z cells induced by CQ was detected by Propyl bromide organism dye. The expression of glucose-regulated protein 78 （GRP-78）,a marker for endoplasmic reticulum stress, in CNE-2Z cells treated with 10 μmol/L of CQ at 0,6,16 and 24 h were detected by Western blot. Results：The different concentration CQ had obviously inhibition effects on CNE-2Z cells. The cell survival rates of CNE-2Z cells treated with 16 μmol/L at 24,48 and 72 h were 85.94% ,75.34% and 56.45% ,respectively. CQ could inhibit the colony formation, induce the apoptosis in a dose-dependent manner and increase the GRP-78 expression of CNE-2Z cells. Conelusions：CQ can inhibit the proliferation of CNE-2Z cells and induce their apoptosis through the overreaction mechanism of endoplasmic reticulum stress.
出处
《蚌埠医学院学报》
CAS
2013年第11期1396-1398,1401,共4页
Journal of Bengbu Medical College
关键词
咽肿瘤
鼻咽癌
氯喹
细胞凋亡
pharyngeal neoplasms
nasopharyngeal carcinoma
chloroquine
apoptosis
