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尿泛素-核糖体融合蛋白52和尿维生素D结合蛋白在糖尿病肾病患者的表达及意义 被引量:3

Urine UbA52 and urine VDBP detection and significance in patients with diabetic nephropathy
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摘要 目的通过检测尿泛素-核糖体融合蛋白52(UbA52)和尿维生素D结合蛋白(VDBP)含量探讨其在糖尿病肾病(DN)诊断中的价值。方法糖尿病肾病患者46例(糖尿病肾病组)、2型糖尿病患者38例(糖尿病组),以及同期在我院体检的健康者40例作为健康对照组。采用酶联免疫吸附测定(ELISA)法检测尿UbA52、尿VDBP,比较尿UbA52、尿VDBP、24小时尿蛋白定量、尿白蛋白与尿肌酐的比值(ACR)、尿UbA52联合尿VDBP对糖尿病肾病诊断的特异性和敏感性。结果3组尿UbA52、尿VDBP差异有统计学意义(P<0.05),其中DN组尿UbA52、尿VDBP明显高于糖尿病组和健康对照组,糖尿病组与健康对照组差异无统计学意义(P>0.05)。尿UbA52、尿VDBP、24小时尿蛋白定量、ACR、尿UbA52联合尿VDBP诊断DN的ROC曲线下面积分别为0.729、0.776、0.629、0.657、0.779。结论检测尿UbA52、尿VDBP水平对于评估糖尿病肾病病情有一定参考价值,联合检测尿UbA52与尿VDBP对于糖尿病肾病的诊断有重要的临床意义。 Objective To explore the value of urine ubiquitin 52 amino acid fusion protein(UbA52)and urine vitamin D binding protein(VDBP)in diabetic nephropathy(DN)diagnosis by detecting urine UbA52 and urine VDBP concentrations.Methods A total of 46 patients with DN and 38 patients with type 2 diabetes mellitus were treated as DN group and diabetic group.ELISA was used to detect UbA52 and VDBP in urine.Analysis of differences between three groups,the differences on DN diagnostic specificity and sensitivity in urine UbA52 and urine VDBP,urinary protein quantitative and urinary creatinine ratio(ACR)were compared.Results Urine UbA52 and urine VDBP of three groups were significantly different from each other(P<0.05).Urine UbA52 and urine VDBP of DN group were significantly higher than those of diabetes group and normal control group.Urine UbA52 and urine VDBP of DN group and normal control group were not significantly different(P>0.05).The area under the ROC curve of urine UbA52,urine VDBP,24-hour urine protein quantification,ACR,urine UbA52 combined urine VDBP in diagnosis of DN were 0.729,0.776,0.629,0.657,0.779,respectively.Conclusion The detection of urine UbA52 and urine VDBP levels have certain reference value in evaluating the condition of DN.The combined detection of urine UbA52 and urine VDBP has important clinical significance in diagnosis of DN.
作者 王江丽 刘茂东 迟雁青 张益民 李英 Wang Jiangli;Liu Maodong;Chi Yanqing;Zhang Yimin;Li Ying(Department of Nephrology,Shijiazhuang First Hospital,Shijiazhuang 050011,China;Department of Nephrology,the Third Hospital of Hebei Medical University,Shijiazhuang 050081,China;Department of Nephrology,the Sixth Affiliated Hospital of Sun Yat-sen University,Guangzhou 510655,China)
出处 《临床荟萃》 CAS 2019年第6期541-544,共4页 Clinical Focus
基金 河北省科技厅惠民工程——UbA52和VDBP在糖尿病肾病早期诊断中的应用研究(16277754D) 河北省政府资助省级临床医学优秀人才项目——UbA52和VDBP在糖尿病肾病早期诊断中的应用研究
关键词 糖尿病肾病 泛素类 维生素D结合蛋白 诊断 diabetic nephropathies ubiquitins vitamin D binding protein diagnosis
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