摘要
目的 探讨盐裂皮肤间接免疫荧光及大疱性类天疱疮（BP）180 NC16a-酶联免疫吸附测定（ELISA）检测在BP诊断中的意义。方法 收集2015年1月至2017年8月在中国医学科学院皮肤病医院用盐裂皮肤间接免疫荧光（IIF-SSS）和BP180 NC16a-ELISA检测BP患者174例和对照组129例血清。其中25例BP患者用直接免疫荧光（DIF）进行检测并与IIF-SSS和BP180 NC16a-ELISA敏感性进行比较。结果 IIF-SSS、BP180 NC16a-ELISA的敏感性分别为93.67%、96.55%；特异性分别为100%、96.12%。IIF-SSS与BP180 NC16a-ELISA相关系数0.147，为弱相关。其中25例BP患者血清学诊断方法（IIF-SSS，BP180 NC16a-ELISA）和DIF敏感性比较差异无统计学意义。结论 BP血清学诊断方法特异性强、敏感性高，值得临床推广应用。
Objective To evaluate the value of indirect immunofluorescence on salt-split skin （IIF-SSS）and bullous pemphigoid 180 NC16a enzyme-linked immunosorbent assay （BP180 NC16a-ELISA） in the diagnosis of bullous pemphigoid（BP）. Methods Serum samples were collected from 174 BP patients and 129 controls, who were enrolled from Institute of Dermatology of Chinese Academy of Medical Sciences and Peking Union Medical College between January 2015 and August 2017, and subjected to IIF-SSS and BP180 NC16a-ELISA. Direct immunofluorescence （DIF） test was performed in 25 cases of BP, and its sensitivity for the diagnosis of BP was compared with that of IIF-SSS and BP180 NC16a-ELISA. Results The sensitivities for IIF-SSS and BP180 NC16a-ELISA were 93.67% and 96.55% respectively, and the specificities for IIF-SSS and BP180 NC16a-ELISA were 100% and 96.12% respectively. IIF-SSS was weakly correlated with BP180 NC16a-ELISA with a correlation coefficient of 0.147. There was no significant difference in the sensitivity between the serological diagnostic methods （IIF-SSS and BP180 NC16a-ELISA） and DIF. Conclusion Serological diagnostic methods show high specificity and sensitivity in the diagnosis of BP, and are worthy of clinical promotion and application.
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
2018年第1期10-13,共4页
Chinese Journal of Dermatology
基金
江苏省自然科学基金（BK20160153）
中国医学科学院医学与健康科技创新工程（CIFMS-2017-I2M-1-017）
北京协和医学院协和青年基金（3332016107）
中央级公益性科研院所基本科研业务费（2016ZX320014）
关键词
类天疱疮
大疱性
诊断
荧光抗体技术
间接
皮肤
酶联免疫吸附测定
Pemphigoid, bullous
Diagnosis
Fluorescent antibody technique, indirect
Skin
Enzyme-linked immunosorbent assay
