摘要
用热分离正常人皮肤制备免疫印迹所需的表皮下大疱病抗原。结果表明,热分离皮肤提取表皮和真皮基底膜带抗原的方法明显缩短实验周期,制备的抗原适用于免疫印迹检测表皮下大疱病的自身抗体。
he subepidermal bullous
diseases antigens used in immunoblotting were made from heat-separated normalhuman skin.
The results showed that the methods of extracting epidermal and dermal basement membrane
zoneantigens by heat-separated skin obviously shortened the experimental time of extracting
antigens.The results sug-gest that the subepidermal bullous diseases antigens extracted from
heat-separated skin is useful to measure au-toantibodies of bullous pemphigoid,epidermolysis
bullous acquisita,bullous systemic lupus erythematosus and lin-ear immunoglobulin A bullous
dermatosis with immunoblotting.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
1994年第6期416-418,共3页
Chinese Journal of Microbiology and Immunology
基金
国家教委博士基金
关键词
热分离皮肤
表皮下大疱病
抗原
免疫印迹
Heat-separated skin
Subepidermal bullous
diseases
Antigens
Immunoblotting