摘要
目的检测微RNA-1290在胰腺癌中的表达,探讨其在胰腺癌侵袭转移中的作用。方法免疫组织化学技术检测胰腺癌组织芯片中微RNA-1290的表达,研究其在胰腺癌侵袭转移中的临床意义;实时定量-PCR技术检测5种胰腺癌细胞系AsPC-1、BxPC-3、Capan-2、Panc-1、MIA PaCa-2中微RNA-1290的表达;用微RNA-1290抑制剂处理对数生长期的胰腺癌细胞系Panc-1和MIAPaCa-2,并用Transwell和细胞划痕实验技术检测胰腺癌细胞的侵袭转移能力;蛋白印记(western blot)检测胰腺癌细胞系中侵袭转移相关蛋白COX-2、MMP-2的表达。结果(1)胰腺癌组织中微RNA-1290的表达显著高于正常胰腺组织以及癌旁组织(均P<0.05)。(2)与胰腺正常上皮细胞HPDE相比,不同胰腺癌细胞系中微RNA-1290的表达量均显著升高(均P<0.05),且微RNA-1290在Panc-1和MIA PaCa-2胰腺癌细胞中的表达量显著高于其他胰腺癌细胞系(均P<0.05)。(3)微RNA-1290抑制剂处理胰腺癌细胞Panc-1和MIA PaCa-2后,其胰腺癌细胞的侵袭和转移能力均显著降低(均P<0.05)。(4)微RNA-1290抑制剂能抑制胰腺癌Panc-1和MIA PaCa-2细胞的侵袭相关蛋白基质金属蛋白酶2(MMP-2)、环氧化酶2(COX-2)的表达。结论胰腺癌细胞系和胰腺癌组织中微RNA-1290可能通过调节MMP-2、COX-2等基因的表达参与胰腺癌细胞的侵袭转移。
Objective To investigate the expression of microRNA-1290 in pancreatic cancer and its role in invasion and metastasis of pancreatic cancer. Methods The expression of microRNA-1290 in pancreatic cancer tissue microarray and pancreatic cancer cell lines (AsPC-1, BxPC-3, Capan-2, Panc-1, and MIA PaCa-2) were detected by immunohistochemistry and QT-PCR. The pancreatic cancer cell lines Panc-1 and MIA PaCa-2 in logarithmic growth phase were treated with microRNA-1290 inhibitor, and the invasion and metastasis ability of pancreatic cancer cells were detected by Transwell and wound healing asssay. Western Blot was used to detect the expression of invasion and metastasis-associated proteins cyclooxygenase 2(COX-2) and matrix metalloproteinase 2(MMP-2) in pancreatic cancer cell lines. Results (1) The expression of microRNA-1290 in pancreatic cancer tissues was significantly higher than that in normal pancreatic tissues and adjacent tissues (P<0.05).(2) Compared with pancreatic normal epithelial cells (HPDE), the expression of microRNA-1290 was significantly higher in different pancreatic cancer cell lines (P<0.05). The expression level of MicroRNA-1290 in Panc-1 and MIAPaCa-2 pancreatic cancer cells was significantly higher than that in other pancreatic cancer cell lines (P<0.05).(3) The number of invasive and metastatic cells was significantly decreased after treatment with microRNA-1290 inhibitor (P<0.05).(4) The expression of MMP-2 and COX-2 were decreased in Panc-1 and MIAPaCa-2 pancreatic cancer cells treated with MicroRNA-1290 inhibitor. Conclusion The expression of MMP-2 and COX-2 may be involved in the invasion and metastasis of pancreatic cancer cell by regulating the expression of microRNA-1290 in pancreatic cancer.
作者
陈自力
马亦飞
潘耀振
喻超
谷化剑
朱昌毫
孙诚谊
Chen Zili;Ma Yifei;Pan Yaozhen;Yu Chao;Gu Huajian;Zhu Changhao;Sun Chengyi(Department of Hepatobiliary Surgery,Affiliated Hospital of Guizhou Medical University,Key Laboratory of Hepatobiliary and Pancreatic Surgery,Guizhou Medical University,Guiyang 550004,Guizhou Province;Department of Otorhinolaryngology,Affiliated Hospital of Guizhou Medical University,Guiyang 550004,Guizhou Province)
出处
《中华肝胆外科杂志》
CAS
CSCD
北大核心
2019年第6期457-461,共5页
Chinese Journal of Hepatobiliary Surgery
基金
贵州省科技厅贵州医科大学联合基金(黔科合(2015)7406)
贵州省科学技术厅-贵阳医学院院士工作站肝胆外科分站(黔科合院士站(2015)4013)
贵州省孙诚谊"肝胆胰脾疾病诊治"导师工作室(黔教研合GZS(2016)09)
贵州省科学技术基金(黔科合J字(2015)2013)
贵州省科学技术厅-贵州医科大学附属医院联合基金(黔科合LH字(2016)7229)
贵州省第四批人才基地基金(黔省专合字(2012)94).
