摘要
目的探讨中药方剂赤白汤对急性肝衰竭小鼠肠道菌群的影响。方法将48只小鼠随机分为正常组、模型组、大剂量中药组、中剂量中药组、小剂量中药组和丽珠肠乐组,每组8只。先给动物生理盐水、中药或丽珠肠乐灌胃8d,采用D-氨基半乳糖/脂多糖腹腔注射诱导急性肝衰竭(ALF)。采用平板活菌计数法检测小鼠粪便肠道菌群。结果模型组小鼠血清ALT水平为(1983.3±629.0)U/L,显著高于正常组[(36.9±7.5)U/L,P<0.001],肝脏指数为(6.7±0.6)%,显著高于正常组[(5.2±0.3)%,P<0.001],大肠杆菌为(8.7±0.5)lgCFU/粪便和肠球菌为(9.5±0.3)lgCFU/粪便,显著高于正常组[(7.6±0.9)lgCFU/粪便,P<0.01和(8.8±0.2)lgCFU/粪便,P<0.001],乳酸杆菌为(9.3±0.3)lgCFU/粪便和双歧杆菌为(10.1±0.4)lgCFU/粪便,显著低于正常组[(10.4±0.5)lgCFU/粪便,P<0.001和(11.2±0.5)lgCFU/粪便,P<0.001];与模型组比,大、中、小剂量中药和丽珠肠乐处理动物肝组织出血、坏死改善,肠道菌群部分恢复。结论中药方剂赤白汤具有改善急性肝衰竭小鼠肝功能和肠道菌群紊乱的作用。
Objective To investigate the effect of Chinese herbal medicine on intestinal microflora in mice with D-galactosamine/LPS-induced acute liver failure(ALF).Methods Forty-eight mice were randomly divided into control,model,high-dose herbal medicine,middle-dose herbal medicine and low-dose herbal medicine,and Lizhuchangle group,with 8 mice in each group.The mice were given intragastric administration once daily of normal saline,herbal or lizhuchangle for 8 days,and then,the model was established by intraperitoneal injection of D-galactoside and lipopolysaccharide.The change of intestinal microflora in each group was detected by viable plate count.Results The liver pathology examination in the model group showed obviously hemorrhage and necrosis,suggesting the model was successfully established;serum ALT level in the model group was(1983.3±629.0)U/L,significantly higher than(36.9±7.5)U/L in the control(P<0.001),the liver index was(6.7±0.6)%,significantly higher than(5.2±0.3)%in the control(P<0.001),the number of escherichia coli was(8.7±0.5)CFU/feces and of enterococcus was(9.5±0.3)CFU/feces,were significantly higher than(7.6±0.9)CFU/feces(P<0.01)and(8.8±0.2)CFU/feces(P<0.001)in the control,while the number of lactobacillus was(9.3±0.3)CFU/feces and lifidobacteria was(10.1±0.4)CFU/feces,significantly lower than(10.4±0.5)CFU/feces(P<0.001)and(11.2±0.5)CFU/feces(P<0.001)in the control;the liver injuries and abnormal changes of intestinal microflora in herbal medicine-intervened groups improved obviously as compared with in the model group(P<0.001).Conclusion The administration of Chinese herbal medicine might improve the liver injuries and restore the abnormal changes of intestinal flora in mice with acute liver failure.
作者
张璇
唐小云
唐春银
石学魁
王惠琦
张晓东
沈海萍
杨春辉
Zhang Xuan;Tang Xiaoyun;Tang Chunyin(Department of Infectious Diseases,Hongqi Hospital,Mudanjiang Medical University,Mudanjiang 157011,Helongjiang Province,China)
出处
《实用肝脏病杂志》
CAS
2019年第4期478-481,共4页
Journal of Practical Hepatology
基金
牡丹江医学院研究生创新科研项目(编号:2017YJSCX-33MY)
关键词
急性肝衰竭
中药方剂
肠道菌群
小鼠
Acute liver failure
Chinese herbal medicine
Intestinal flora
Mice
