摘要
试验以600份不同血缘的玉米自交系茎秆为材料,利用近红外光谱仪分别对茎秆中酸性洗涤纤维(ADF)、中性洗涤纤维(NDF)、酸性洗涤木质素(ADL)、纤维素(CEL)、半纤维素(HCEL)、体外干物质消化率(IVDMD)等6个品质性状及与倒伏相关的3个性状茎秆茎粗(SD)、穿刺强度(RPR)、抗弯折能力(S)进行了测定与分析,并对细胞结构进行了扫描电镜观察。结果表明:供试材料中6个与茎秆资源化利用相关的性状ADF、NDF、CEL、ADL、HCEL及IVDMD均存在广泛的遗传差异。IVDMD的变化范围最大为12.04%~58.77%,ADL的变化范围最小为1.06%~6.93%。IVDMD与其他5个品质性状间均存在显著或极显著负相关关系。NDF和ADL对IVDMD的影响较大,二者合计可以解释56.18%的表型变异,茎粗、纤维素和半纤维素对茎秆消化率的影响较小。与倒伏相关的3个性状茎粗、抗弯折能力及穿刺强度经测定发现,倒伏率与茎粗、抗弯折能力及穿刺强度均成极显著负相关关系。抗弯折能力变幅、变异系数均为最大,分别为15.10%~93.80%和29.36%。回归分析结果显示,抗弯折能力对倒伏率的贡献达到极显著水平,抗弯折能力可以解释25%的表型变异。经扫描电镜观察发现,品质性状差异显著的自交系茎秆,其细胞结构特征差异明显。本研究为玉米茎秆饲料化、能源化及抗倒伏育种提供了材料基础和理论依据。
In this study, 600 different maize inbred stalks were used as test materials for analyzing their stalk quality traits and functions. 6 quality traits, including acidic washing fiber(ADF), neutral detergent fiber(NDF), acid washing lignin(ADL), cellulose(CEL), hemicellulose(HCEL), and in vitro dry matter digestibility(IVDMD), and 3 lodging-related traits including stalk diameter(SD), puncture strength(RPR), and bending resistance(S) were measured and analyzed using near-infrared spectroscopy. The cell structure was observed by scanning electron microscopy. The results showed that there were extensive genetic differences among the six traits related to stalk resource utilization, which were ADF, NDF,CEL, ADL, HCEL and IVDMD. The range of IVDMD varies from 12.04% to 58.77%, and the range of ADL varies from1.06% to 6.93%. There was a significant or extremely significant negative correlations between IVDMD and the other five quality traits. NDF and ADL have a greater impact on IVDMD. The combination of the two can explain 56.18% of phenotypic variation. Stem thickness, cellulose and hemicellulose have little effect on stem digestibility. The stems, bending resistance and puncture strength of the three traits related to lodging were measured and analyzed, and it was found that the lodging rate had extremely significant negative correlation with stem diameter, bending resistance and puncture strength.The amplitude and coefficient of variation of resistance to bending were all the largest, which were 15.10%-93.80% and29.36% respectively. The regression analysis showed that the contribution of the bending resistance to the lodging rate reached a very significant level, and the bending resistance ability explained 25% of the phenotypic variation. Scanning electron microscopy showed that the cell structure characteristics of the inbred stalks with significant differences in quality traits were significantly different. The research provides a material basis and theoretical basis for corn stalk feed, energy and lodging resist
作者
张孝林
占继敏
王丽
吴子温
吴鹏冲
范锦胜
张凤云
路运才
ZHANG Xiaolin;ZHAN Jimin;WANG Li;WU Ziwen;WU Pengchong;FAN Jinsheng;ZHANG Fengyun;LU Yuncai(College of Agricultural Resources and Environment, Heilongjiang University, Harbin 150080, China)
出处
《河北农业大学学报》
CAS
CSCD
北大核心
2019年第3期8-13,共6页
Journal of Hebei Agricultural University
基金
“十三五”国家重点研发计划项目(2016YFD0101803)
黑龙江大学2011协同创新项目“能源型玉米种质资源的创制与利用”
关键词
玉米自交系
茎秆
品质分析
倒伏
电镜观察
maize inbred lines
corn stalks
quality trait
lodging
scanning electron microscope
