摘要
目的对C57BL/6 ApoE^-/-小鼠股动脉采用部分结扎的方法制作小鼠后肢动脉狭窄模型,血管造影对小鼠后肢动脉狭窄率进行评估。方法16只ApoE^-/-麻醉小鼠后钝性分离出右侧股动脉,平行股动脉放置金属丝,细线同时捆绑股动脉和金属丝,结扎后抽出金属丝,缝合皮肤伤口,对侧同法处理但不结扎;高脂饮食喂养4周后处死小鼠暴露心脏,从左心室手推造影剂,放入小动物X线成像系统进行血管造影;造影后留取小鼠双侧股动脉组织,结扎侧股动脉称为实验组(n=16),未结扎侧为对照组(n=16),应用SPSS 23.0进行统计分析,计量资料以均数±标准差表示,等级资料采用秩和检验证,率的比较采用χ^2检验,组间比较采用单因素方差分析。结果实验组有13个标本有斑块,对照组有4个标本有斑块;使用血管造影对小鼠后肢动脉进行影像学评估统计结果显示,16周龄C57BL/6ApoE^-/-小鼠股动脉狭窄率测量值为(48.43±25.13)%,狭窄率理论值为[(45.63±9.96)%,F=7.956,P>0.05],左侧股骨头平面小鼠股动脉直径为(0.97±0.11)mm。结论股动脉捆绑结扎法是小鼠后肢动脉狭窄模型制作的一种可靠方法,血管造影可评估小鼠后肢动脉狭窄率。
Objective The femoral artery of C57BL/6 ApoE^-/- mice was ligated to make the model of hind-limb artery stenosis. Angiography was used to evaluate the stenosis rate of hind-limb artery in mice. Methods The ligated femoral arteries were divided into the experimental group and the contralateral femoral arteries were the control group. We exposed the right femoral artery in the operative field using blunt dissection technique, parallel placed the metal wire with femoral artery, used thread to bound the femoral artery and the metal wire, drawn out the metal wire after ligation, and then sutured the skin wound. The contralateral was dealt with the same method but not ligated. After 4 weeks of high fat diet, the mice were killed to expose their hearts, and the contrast medium was pushed from the left ventricle, then put into the X-ray imaging system of small animals for angiography. The bilateral femoral arteries were taken from the mice after angiography. Results There were 13 plaques in the experimental group and 4 in the control group. The angiography results showed that the femoral artery stenosis rate of 16-week-old C57BL/6 ApoE^-/- mice model was measured to be (48.43±25.13)%, the theoretical stenosis rate was (45.63±9.96)%, F=7.956, P>0.05. The diameter of femoral artery was (0.97±0.11) mm. Conclusion The method of femoral artery ligation is a reliable method to the establishment of the model of hind-limb artery stenosis in C57BL/6 ApoE^-/- mice and angiography is one of the methods to evaluate the rate of stenosis.
作者
蔡申华
胡杰
雷彤彤
田琴琴
师佩璐
邢森
张永吉
齐炳才
袁萌
张智鑫
董红霖
Cai Shenhua;Hu Jie;Lei Tongtong;Tian Qinqin;Shi Peilu;Xing Miao;Zhang Yongji;Qi Bingcai;Yuan Meng;Zhang Zhixin;Dong Honglin(Department of Vascular Surgery,the Second Clinical Medicine School of Shanxi Medical University,Taiyuan 030001,China;Department of Nephrology,Shanxi Provincial People’s Hospital,Shanxi Medical University,Taiyuan 030012,China)
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2019年第6期1128-1131,共4页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金(8187021553)
山西省回国留学人员基金(2016-053).
关键词
股动脉狭窄
模型
动物
动脉造影
股动脉斑块
Femoral artery stenosis
Model,animal
Angiography
Femoral artery plaque
