摘要
目的研究金丝草Pogonatherum crinitum(Thunb.) Kunth的化学成分及其体外抗HBV活性。方法金丝草70%乙醇提取物正丁醇部位采用大孔树脂、硅胶、聚酰胺、Sephadex LH-20及HPLC进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。HepG2.2.15细胞模型检测体外抗HBV活性。结果从中分离得到8个化合物,分别鉴定为5-羟基-4′-甲氧基黄酮-7-O-芸香糖苷(1)、1,7-二羟基-3,8-二甲氧基呫吨酮(2)、芹菜素-7-O-β-D-(6″-E-p-香豆酰基)-葡萄糖苷(3)、大豆素-7,4′-二-O-葡萄糖苷(4)、5,7,3′-三羟基-4′-甲氧基黄酮-3-O-芸香糖苷(5)、N-乙酰苯丙氨酸(6)、焦谷氨酸甲酯(7)、杜鹃花酸(8)。化合物6对HepG2.2.15细胞分泌表面抗原(HBsAg)及e抗原(HBeAg)有一定的抑制作用,IC50分别为55.5、69.5μg/mL。结论所有化合物均为首次从金发草属植物中分离得到。化合物6有一定的体外抗HBV活性。
AIM To study the chemical constituents from Pogonatherum crinitum(Thunb.) Kunth and their anti-HBV activities in vitro.METHODS The n-butyl fraction of 70% ethanol extract was isolated and purified by macroporous resin, silica,polyamide,Sephadex LH-20 and HPLC,then the structures of obtained compounds were identified by physicochemical properties and spectral data.The anti-HBV activities in vitro were tested in the model of HepG2.2.15 cells.RESULTS Eight compounds were isolated and identified as 5-hydroxy-4′-methoxyflavone-7-O-rutinoside(1), 1,7-dihydroxy-3,8-dimethoxyxanthone(2), apigenin-7-O-β-D-(6″-E-p-coumaroyl)-glucopyranoside(3), daidzein-7,4′-O-diglucopyranoside(4), 5,7,3′-trihydroxy-4′-methoxyflavone-3-O-rutinoside(5),N-acetyl phenylalanine(6), L-pyroglutamic acid methyl ester(7), azelaic acid(8). Compound 6 had certain inhibitory effects on HBsAg and HBeAg with the IC50 values of 55.5,69.5 μg/mL, respectively.CONCLUSION All the compounds are isolated from genus Pogonatherum for the first time.Compound 6 has certain anti-HBV activities in vitro.
作者
王慧娜
尹志峰
尹鑫
李洪波
赵桂琴
WANG Hui-na;YIN Zhi-feng;YIN Xin;LI Hong-bo;ZHAO Gui-qin(Hebei Provincial Key Laboratory of Research and Development for Traditional Chinese Medicine,Institute of Chinese Mateia Medica of Chengde Medical College,Chengde 067000,China;Chengde Academy of Agriculture and Forestry Science,Chengde 067000,China)
出处
《中成药》
CAS
CSCD
北大核心
2019年第6期1308-1312,共5页
Chinese Traditional Patent Medicine
基金
河北省教育厅重点项目(ZD2016061)
河北省中医药管理局项目(2015034)
承德医学院科学研究重点项目(201606)
河北省高校重点学科建设项目(冀教高[2013]4)
关键词
金丝草
化学成分
分离鉴定
生物活性
体外
Pogonatherum crinitum(Thunb.) Kunth
chemical constituents
isolation and identification
biological activities
in vitro
