摘要
目的观察Genistein对雌性大鼠卵巢颗粒细胞卵泡刺激素受体(FSHR)基因表达的影响,及其与雌激素受体(ER)的关系。方法选取25~28日龄雌性大鼠,皮下注射孕马血清促性腺激素,建立促卵泡发育模型,分离卵巢颗粒细胞进行培养,分别给予Genistein(0、0.1、1、5、10、100μmol/L)及雌激素受体阻断剂ICI182,780(1μmol/L)处理24h后,采用逆转录-聚合酶链反应(RT-PCR)检测芳香化酶基因上游调控因子FSHR mRNA的表达。结果与空白对照组比较,10μmol/LGenistein组FSHR mRNA表达显著增加(P<0.05)。ICI 182,780(1μmol/L)预处理细胞30min后加入1和10μmol/L Genistein,其FSHR mRNA表达分别较1和10μmol/L Genistein组显著下降(P<0.05或P<0.01)。结论10μmol/L的Genistein可上调雌性大鼠卵巢颗粒细胞中FSHR表达,且该作用可能是通过ER介导的。
Objective To observe the effect of Genistein on the expression of follicle stimulating hormone receptor(FSHR)gene in ovarian granulosa cells of female rats and its relationship with estrogen receptor(ER).Methods Female rats aged 25-28 days were subcutaneously injected with pregnant mare serum gonadotrophin to establish follicular development model.Ovarian granulosa cells were isolated and cultured.Genistein(0,0.1,1,5,10,100μmol/L)and estrogen receptor blocker ICI 182,780(1μmol/L)were treated for 24 hours respectively.Reverse transcription-polymerase chain reaction(RT-PCR)was used to detect the expression of FSHR mRNA,an upstream regulator of aromatase gene.Results Compared with the blank control group,the expression of FSHR mRNA was significantly increased in the 10μmol/L Genistein group(P<0.05).After ICI 182,780(1μmol/L)pretreatment for 30 minutes,the expression of FSHR in ICI 182,780 pretreated cells with 1 and 10μmol/L Genistein was significantly lower than that in the 1 and 10μmol/L Genistein groups(P<0.05 or P<0.01)respectively.Conclusion 10μmol/L of Genistein can up-regulate the expression of FSHR in ovarian granulosa cells of female rats,and this effect may be mediated by ER.
作者
温海霞
张亚楠
WEN Haixia;ZHANG Ya′nan(Department of Physiology,Basic Medical College,Harbin Medical University,Heilongjiang Province,Harbin 150086,China)
出处
《中国医药导报》
CAS
2019年第14期16-18,共3页
China Medical Herald
基金
黑龙江省留学归国基金项目(LC2013C06)
