摘要
目的 观察大豆异黄酮（SI）及其主要活性成分genistein对衰老大鼠卵巢及体外培养的卵巢颗粒细胞中雌激素受体α（ER-α）表达的影响,初步探讨SI作用于卵巢的分子机制.方法 自然老化法建立围绝经期大鼠动物模型,分别给予低剂量（50 mg/kg）、中剂量（158 mg/kg）、高剂量（500 mg/kg）的SI灌胃处理8周.采用反转录-聚合酶链反应（RT-PCR）和免疫组化SP法分别检测卵巢组织中ER-α mRNA和蛋白的表达.离体培养大鼠颗粒细胞24 h后,给予genistein（0、0.1、1、5、10、100 μmol/L）继续处理细胞48 h,采用RT-PCR方法检测卵巢颗粒细胞中ER-α mRNA的表达.结果 低、中、高剂量SI组卵巢ER-α mRNA表达强度分别为0.207±0.014、0.316±0.073和0.402±0.170,均显著高于模型组0.062±0.005（均P〈0.01）.低、中、高剂量SI组卵巢ER-α蛋白的表达强度分别为7.35±4.90、13.90±5.12和23.79±10.31,均显著高于模型组2.74±0.09（均P〈0.01）.1、5、10 μmol/L的genistein作用卵巢颗粒细胞48 h,ER-α mRNA表达量分别为0.927±0.232、1.067±0.154、1.118±0.126,均显著高于对照组0.671±0.170（均P〈0.01）,而100 μmol/L genistein组ER-α mRNA的表达量为0.573±0.210,低于对照组（P〈0.05）.结论 一定剂量的SI可明显上调衰老卵巢ER-α mRNA和蛋白的表达.SI主要活性成分genistein,可调节体外培养大鼠卵巢颗粒细胞中ER-α mRNA的表达,其作用可能与浓度有关（1～10 μmol/L 的genistein对ER-α基因表达起上调作用）.
Objective To investigate the effects of soy isoflavones （SI） on the expression of estrogen receptor-α （ER-α） in senile rat ovaries and ovarian granulosa cell cultured in vitro treated with genistein, a major active component of SI. Methods The animal model of perimenopause rats was established by unforced aging. The animals were treated by intragastric administration （ig） with low （50 mg/kg）, middle （158 mg/kg） and high （500 mg/kg） dose of SI for 8 weeks. The expressions of ER-α mRNA and protein were detected by reverse transcription-polymerase chain reaction （RT-PCR） and immunohistochemistry respectively. The granulosa cells of rat ovaries were isolated and administered with genistein（0, 0.1, 1, 5, 10, 100 μmol/L） for 48 h and the expression levels of ER-α mRNA detected by RT-PCR. Results The ER-α mRNA expression levels of the low, middle and high dose groups of SI （0.207±0.014, 0.316±0.073 and 0.402±0.170 respectively） were higher than those of the model group （0.671±0.170） （all P〈0.01）. The expression levels of ER-α protein for the low, middle and high dose groups of SI （7.35±4.90, 13.90±5.12 and 23.79±10.31 respectively） were higher than those of the model group （2.74±0.09） （all P〈0.01）. The expression levels of ER-α mRNA in granulosa cells treated with 1, 5, 10 μmol/L genistein for 48 h were 0.927±0.232, 1.067±0.154, 1.118±0.126 respectively（all P〈0.01）. They were higher than those of the control group （0.671±0.170）. But the expression levels of 100 μmol/L genistein group were lower than those of the control group （P〈0.05）. Conclusion Soy isoflavones can up-regulate the expressions of ER-α mRNA and protein in senile rat ovaries. As a major active component of soy isoflavones, genistein can regulate the expressions ER-α mRNA in granulosa cells of rat ovaries. Such an effect is concentration-dependent. And 1-10 μmol/L genistein may up-regulate the expression of ER-α mRNA.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2011年第28期1987-1991,共5页
National Medical Journal of China
基金
基金项目：中国博士后基金（20080430944）
黑龙江省青年科学基金（QC07C63）
黑龙江省博土后基金（LRB07-280）
