摘要
为明确贵州省白及叶褐斑病病原菌及其生物学特性,采用组织分离法和离体接种法分别对其病原菌进行分离和致病性测定,利用形态学及ITS、EF-1α、β-tubulin基因序列分析对病原菌的分类进行鉴定,并对病原菌的生物学特性进行研究。结果表明:分离得到的白及叶褐斑病病原菌菌株编号为HGUP_(17)355;该菌菌丝白色、羊绒状,菌落中心隆起呈淡黄色、背面枫叶红色,大型分生孢子窄镰刀形,3～8个分隔,未见厚垣孢子,小型分生孢子卵圆形,1～2个分隔;在以ITS、EF-1α、β-tubulin三个基因序列构建的系统发育树中,菌株HGUP_(17)355与燕麦镰刀菌Fusarium avenaceum聚于一支,且支持率达100%,结合形态特征与分子生物学将其鉴定为燕麦镰刀菌。该菌菌丝最适生长温度为20℃,最适生长pH为6,在燕麦片琼脂培养基上生长最快,以麦芽糖为碳源、以L-苯丙氨酸为氮源时利用率最高,致死温度为45℃,对光照不敏感。
To clarify the pathogen causing leaf brown spots of Chinese ground orchid Bletilla striata in Guizhou Province and its biological characteristics, the pathogen was obtained by using the method of tissue isolation. Its pathogenicity was tested in vitro inoculated leaves, and the pathogen was classified and identified with morphology and phylogeny based on combined ITS, EF-1α and β-tubulin sequence analysis;the biological characteristics of pathogen were also studied. The results showed that the strain isolated from leaf brown spots of B. striata was named as HGUP17355. Its main diagnostic features were as followed: white mycelia, cashmere-like form, uplifted center of colonies, faint yellow, maple-leaf red dorsal surface, oval microconidia with one-two septa, narrow sickle-like macroconidia with three-eight septa, and no chlamydospores. The phylogenetic tree based on combined ITS, EF-1α, β-tubulin sequences showed that HGUP17355 strain was clustered together with Fusarium avenaceum, supported by 100%. According to the combined morphological characteristics and phylogenetic tree, the pathogen was identified as F. avenaceum. The mycelia of HGUP17355 strain grew fastest on oatmeal agar medium with maltose and L-phenylalanine as the carbon and nitrogen sources, respectively, at 20℃, pH 6.0, and its fatal temperature was 45℃ and it was insensitive to light.
作者
宋莉莎
曾桂萍
任静
司世飞
赵致
李忠
Song Lisha;Zeng Guiping;Ren Jing;Si Shifei;Zhao Zhi;Li Zhong(College of Agriculture,Guizhou University,Guiyang 550025,Guizhou Province,China)
出处
《植物保护学报》
CAS
CSCD
北大核心
2019年第2期337-344,共8页
Journal of Plant Protection
基金
贵州省科技支撑计划(黔科合支撑[2017]2828)
科技部科技基础性工作专项(2014FY120100)
贵州省中药材现代农业产业体系建设项目(GZCYTX2015-0202)
关键词
白及
叶褐斑病
病原菌鉴定
燕麦镰刀菌
生物学特性
Bletilla striata
leaf brown spot
pathogen identification
Fusarium avenaceum
biological characteristics
