摘要
目的探索miR-143-3p在胃癌细胞和胃癌组织中的表达、并分析其异常表达的临床意义;同时采用生物信息学方法,进行靶基因和信号通路富集分析。方法采用实时荧光定量PCR法检测miR-143-3p在正常胃黏膜细胞和5种胃癌细胞,以及胃癌组织和癌旁组织中的表达,并分析其临床意义。采用OncomiR和YM500数据库分析miR-143-3p在胃癌组织和癌旁组织中的表达。采用miRecords网站数据库的预测软件预测其靶基因,选择至少3个以上软件支持的靶基因,运用DAVID 6.7软件在线富集分析其靶基因参与的信号通路。结果实验结果表明:相对于正常胃黏膜上皮细胞GES-1,miR-143-3p在不同分化程度的胃癌细胞,包括SGC-7901(中分化)、MKN-45(中分化)、MGC-803(低分化)、BGC-823(低分化)和HGC-27(未分化)细胞中的表达量均明显下调(P<0.001)。实时荧光定量PCR检测结果表明:相对于癌旁组织,miR-143-3p在胃癌组织中的表达在36例中下调,在18例中上调,4例变化不明显。miR-143-3p在胃癌组织中的表达与胃癌淋巴结转移以及浸润深度相关(P<0.05)。生信分析结果显示,miR-143-3p的靶基因富集在38个与肿瘤相关的信号通路中。结论 miR-143-3p是胃癌中表达下调的分子标志物,是潜在的具有临床相关性的抑癌基因,可能参与胃癌发生发展过程中的生物学行为。
Objective To observe the expressions of miR-143-3 p in gastric cancer cells and gastric carcinoma tissues with its clinical significance, and to analyze the target genes with enriched pathway by using bioinformatics methods. Methods The expressions of miR-143-3 p in different differentiation gastric cancer cells and normal gastric mucosa cell line, and the expressions in gastric cancer tissues and adjacent tissues were detected by real-time fluorescent quantitative PCR. In addition, OncomiR and YM500 databases were used to analyze the expression of miR-143-3 p in gastric cancer tissues compared with adjacent tissues. Furthermore, the targets of miR-143-3 p were predicted by using the software of miRecords website database, and at least three software-supported target genes were chosen to analyze the enriched the signal pathways in which the target gene was involved with DAVID 6.7 software. Results The expressions of miR-143-3 p in the different differentiation degree of gastric cancer cells compared with normal gastric mucosa cell line were downregulated(P<0.001), and the expression of miR-143-3 p in gastric cancer tissues compared with adjacent tissues was also downregulated(downregulated in 36 cases, upregulated in 18 cases, and no alteration in 4 cases). The expression of miR-143-3 p in gastric cancer tissues was associated with lymph node metastasis and invasion depth(P<0.05).Bioinformatics analysis results showed that the target genes of miR-143-3 p were enriched in 38 signaling pathways associated with cancer. Conclusion MiR-143-3 p is a down-regulated molecular marker in gastric cancer and a potentially clinically related tumor suppressor gene, which may be involved in the cancerous phenotype in carcinogenesis and development of gastric cancer.
作者
牛荣
王转兄
白悦
赵文涛
呼永华
田一虹
李海龙
NIU Rong;WANG Zhuanxiong;BAI Yue;ZHAO Wentao;HU Yonghua;TIAN Yihong;LI Hailong(No.1 Department of Thoracic Surgery, Gansu Provincial Cancer Hospital, Lanzhou 730050, P. R. China;School of Clinical Medicine, Gansu University of Chinese Medicine, Lanzhou 730000, P. R. China)
出处
《中国普外基础与临床杂志》
CAS
2019年第5期538-544,共7页
Chinese Journal of Bases and Clinics In General Surgery
基金
甘肃省高等学校科研项目(项目编号:甘财教[2014]63-15)
甘肃省高等学校科研项目计划(项目编号:2016A-045)
