摘要
目的:探讨E-钙粘蛋白(E-cadherin)和S100钙结合蛋白A4(S100A4)在乳腺癌组织中的表达及其与病理特征的关系。方法:选取2010年3月-2017年12月期间梅州市人民医院收集的乳腺癌石蜡块标本50例为乳腺癌组,另选取同时期病理检查为良性肿瘤的石蜡块标本38例为对照组,应用免疫组化法检测E-cadherin和S100A4在乳腺癌组与对照组的表达情况,分析E-cadherin和S100A4与乳腺癌病理特征的关系,并采用Spearman相关性分析方法分析E-cadherin和S100A4的相关性。结果:E-cadherin在乳腺癌组中的阳性表达率低于对照组,S100A4阳性表达率高于对照组(P<0.05)。E-cadherin和S100A4阳性表达率与乳腺癌患者的年龄、是否绝经及病灶直径无关(P>0.05),有淋巴结转移、临床分期为Ⅲ-Ⅳ期、病理分级为Ⅲ级的乳腺癌患者中E-cadherin阳性表达率低于无淋巴结转移、临床分期为Ⅰ-Ⅱ期、病理分级为Ⅰ-Ⅱ级者,而有淋巴结转移、临床分期为Ⅲ-Ⅳ期、病理分级为Ⅲ级的乳腺癌患者中S100A4阳性表达率高于无淋巴结转移、临床分期为Ⅰ-Ⅱ期、病理分级为Ⅰ-Ⅱ级者(P<0.05)。经Spearman相关性分析显示,E-cadherin阳性表达与S100A4阳性表达呈负相关(P<0.05)。结论:S100A4在乳腺癌组织中呈现高表达,E-cadherin乳腺癌组织中呈现低表达,且其与患者淋巴结转移、临床分期及病理分级存在一定的相关性。
Objective: To investigate the expression levels of E-cadherin (E-cadherin) and S100 calcium binding protein A4 (S100A4) in breast cancer tissues and its relationship with pathological features. Methods: A total of 50 cases of breast cancer specimens from Meizhou People's Hospital from March 2010 to December 2017 were selected as breast cancer group, and 38 cases of benign paraffin block specimens from pathological examination during the same period were selected as control group. Immunohistochemical method was used to detect the expression of E-cadherin and S100A4 in breast cancer group and control group. The relationship between E-cadherin and S100A4 and pathological characteristics of breast cancer was analyzed. The correlation between E-cadherin and S100A4 was analyzed by Spearman correlation analysis method. Results: The positive expression rate of E-cadherin in breast cancer group was lower than that in control group,and the positive expression rate of S100A4 in breast cancer group was higher than that in control group (P<0.05). The positive expression rates of E-cadherin and S100A4 were independent of the age, whether menopause and lesion size of breast cancer patients (P>0.05). The positive rate of E-cadherin expression in breast cancer patients with lymph node metastasis, Ⅲ-Ⅳ stage of clinical stage and Ⅲ grade of pathological grade was lower than that without lymph node metastasis, Ⅰ-Ⅱ stage of clinical stage and Ⅰ-Ⅱ grade of pathological grade, the positive expression rate of S100A4 of the former was higher than that of the latter (P<0.05). Spearman correlation analysis showed that the positive expression of E-cadherin was negatively correlated with the positive expression of S100A4 (P<0.05). Conclusion: The expression of S100A4 in breast cancer tissues is high and the expression in E-cadherin breast cancer tissues is low, and there is a certain correlation between lymph node metastasis, clinical stage and pathological grading.
作者
张思明
李洪胜
柳玉梅
金田恩
彭聪
ZHANG Si-ming;LI Hong-sheng;LIU Yu-mei;JIN Tian-en;PENG Cong(Department of Breast surgery,Meizhou Hospital Affiliated to Sun Yat-sen University/Meizhou People's Hospital,Meizhou,Guangdong,514000,China;Department of Breast surgery,Cancer Hospital Affiliated to Guangzhou Medical University,Guangzhou,Guangdong,510095,China;Department of Pharmacology,Medical College of Jiaying University,Meizhou,Guangdong,514000,China;Department of Pathology,Guangzhou Da An Clinical Laboratory Center,Guangzhou,Guangdong,510520,China;Department of Pathology,Cancer Hospital Affiliated to Guangzhou Medical University,Guangzhou,Guangdong,510095,China)
出处
《现代生物医学进展》
CAS
2018年第22期4308-4311,共4页
Progress in Modern Biomedicine
基金
广东省自然科学基金项目(2017A030313551)
