摘要
【目的】探讨调胃承气汤对肠源性脓毒症(GOS)大鼠肠黏膜屏障功能的保护作用及机制。【方法】将40只SD雄性大鼠随机分为假手术组,模型组,乌司他丁组,中药高、低剂量组,每组8只。除假手术组外,其余大鼠均采用盲肠结扎穿刺法(CLP)复制GOS大鼠模型。造模结束后2、10、24 h,中药高、低剂量组大鼠分别给予调胃承气汤(剂量分别为9.45、4.73 g/kg)灌胃,乌司他丁组大鼠给予腹腔注射乌司他丁(剂量为30 000 U/kg),假手术组与模型组给予等体积生理盐水灌胃。最后一次给药0.5 h后,采用酶联免疫吸附分析(ELISA)检测血清中白细胞介素6(IL-6)、内毒素(LPS)和肠型脂肪酸结合蛋白(iFABP)的含量。采用苏木素—伊红(HE)染色法观察小肠组织病理结构变化,免疫组化法检测小肠组织occludin蛋白的表达。【结果】与假手术组比较,模型组血清IL-6、LPS、iFABP水平显著提高(P <0.01),小肠组织occludin蛋白表达显著降低(P <0.01)。与模型组比较,乌司他丁组、中药高剂量组和中药低剂量组血清IL-6、LPS、iFABP水平显著降低(P <0.05或P <0.01),小肠组织occludin蛋白表达显著升高(P <0.01)。病理学观察发现,模型组小肠绒毛、黏膜间隙、固有层等结构产生严重异常,乌司他丁组、中药不同剂量组绒毛结构、黏膜间隙、固有层等结构相对完整。【结论】调胃承气汤可能通过上调小肠组织紧密连接蛋白occludin的表达,从而抑制细菌与内毒素引起炎性反应造成肠黏膜组织结构的损伤,进而对GOS大鼠肠黏膜屏障功能起到一定的保护作用。
Objective To explore the protective effects of Tiaowei Chengqi Decoction on intestinal mucosal barrier function in rats with gut-origin sepsis(GOS)and its mechanism. Methods Forty SD male rats were randomly divided into sham operation group, model group, ulinastatin group, high-and low-dose Chinese medicine groups,8 rats in each group. Except for the sham operation group,the rats in other groups were induced to GOS model by cecal ligation puncture(CLP). At 2, 10, 24 h after modeling, the high-and low-dose Chinese medicine groups were given intragastric administration of Tiaowei Chengqi Decoction(at the dosage of 9.45,4.73 g/kg,respectively),the ulinastatin group was given intraperitoneal injection of ulinastatin(at prescription amount of 30 000 U/kg),and the sham operation group and model group were given the same volume of normal saline. The experiment was terminated 0.5 h after the last administration,and then the serum levels of interleukin 6(IL-6),endotoxin(LPS)and intestinal fatty acid binding protein(iFABP)were measured by enzyme-linked immunosorbent assay(ELISA). The pathological changes of small intestine tissues were observed by hematoxylin-eosin(HE)staining. The expression of occludin protein in the small intestine was detected by immunohistochemistry. Results Compared with the sham operation group,the serum levels of IL-6,LPS and iFABP in the model group were significantly increased(P < 0.01), and the expression level of occludin protein in the small intestine was significantly decreased(P < 0.01). Compared with the model group,the serum levels of IL-6,LPS and iFABP were significantly lowered(P < 0.05 or P < 0.01), and the expression level of occludin protein in the small intestine was significantly increased(P < 0.01)in the ulinastatin group,high-and low-dose Chinese medicine groups. The pathological results showed that the structure of small intestinal villi,mucosal space,lamina propria and other structures in the model group were severely damaged,while the structures of villi,mucosal space andlamina
作者
张延林
郑刚
熊明
王昊
易绍龙
努尔阿依木.莫合塔
依不拉音.库尔班
黄虎
赵锋利
ZHANG Yan-Lin;ZHENG Gang;XIONG Ming;WANG Hao;YI Shao-Long;MUHETAER Nuerayimu;KU ERB AN Yibulayin;HUANG Hu;ZHAO Feng-Li(The First People's Hospital of Kashi District of Xinjiang,Kashi 844000 Xinjiang,China;The First Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510405 Guangdong,China)
出处
《广州中医药大学学报》
CAS
2019年第6期874-878,共5页
Journal of Guangzhou University of Traditional Chinese Medicine
基金
新疆维吾尔自治区自然科学基金联合基金资助项目(编号:2016D01C020)
