摘要
目的:观察腹腔感染状态下大鼠肠黏膜形态学和通透性的变化,观察肠源性内毒素体内移位途径,探讨清热解毒方剂肠屏障保护作用的机制。方法:采用人工胃液联合大肠杆菌腹腔内注射的方法建立大鼠腹腔感染模型。将Wistar大鼠分为3组:正常对照组(C组)、模型组(M组)、清热解毒方剂组(QRJD组),分别观察造模后6h,24h,72h回肠的病理学改变以及尿液中乳果糖与甘露醇的比值(L/M)的变化。采用荧光化学发光法检测门静脉、乳糜管淋巴液、腹主动脉血以及肠系膜淋巴结、肝、肺、肠、脾组织FITC-LPS荧光强度。结果:模型组肠黏膜损伤,尿液L/M显著升高,脏器组织中FITC-LPS含量由高至低为:肠系膜淋巴结、肺、肝、肠、脾,体液中FITC-LPS含量以乳糜管淋巴液含量最高。清热解毒方剂组上述指标较模型组明显改善。结论:清热解毒方剂可以显著减轻大鼠腹腔感染所致肠黏膜损伤,改善肠黏膜通透性,减少肠源性内毒素移位,改善肠道屏障功能。
Objective To investigate the effect of QlngReJleDu （清热解毒）（QRJD） herbal decoction on the gut mucosal barrier function of rats with acute bacterial peritonitis. Methods The rat model of sepsis was prepared by in- tra- abdominal E. coli administration. The histomorphology of the ileum and Lactose/Mannitol（L/M） ratio in urine were observed at 6 h, 24 h and 72 h after the model formation. The concentrations of FITC - LPS in the portal vein blood, the thoracic duct lymph, the aortal blood and the tissue samples of the mesenteric lymph node（MLN）, liver lung, intestine and spleen were detected at 24 h. Results The intestinal mucosal barrier showed obvious pathological lesions, the ra- tio of L/M increased in the model group during abdominal infection. The FITC - LPS concentration was higher in the MLN, lung, liver and thoracic duct than those in other tissue. The indicators of the QRJD group were better than those of the model group. Conclusion Abdominal infection can lead to the damage of gut mucosal barrier function and translocation of intestinal endotoxin. QRJD herbal decoction could protec the gut barrier function and significantly inhibit the translocation of gut - derived endotoxin in rats with peritonitis.
出处
《中国中西医结合外科杂志》
CAS
2009年第3期305-308,共4页
Chinese Journal of Surgery of Integrated Traditional and Western Medicine
基金
天津市科委重大科技攻关项目(O5yfgdsf02600)
关键词
腹腔感染
肠黏膜通透性
肠源性内毒素移位
清热解毒方剂
abdominal infection, intestinal mucosal permeability, gutderived endotoxin translocation, QingRe- Jiedu herbal decoction.
