摘要
目的检测β-连环蛋白(β-catenin)和生存素(survivin)蛋白在老年结直肠腺癌组织中的表达,分析其与老年结直肠腺癌患者性别、淋巴结转移以及肿瘤-淋巴结-转移(TNM)分期之间的关系,探讨β-catenin和survivin蛋白表达的相关性。方法收集2015年1月至2017年12月在长治医学院附属和平医院及和济医院进行手术切除治疗的结直肠腺癌患者50例,将外科手术切除后的标本分为2组:癌组织组和正常组织组(距离癌组织边缘>2 cm,病理检测结果正常),每组50例。采用免疫组织化学法检测β-catenin和survivin蛋白表达。采用SPSS 22.0软件进行数据处理。组间比较采用Mann-Whitney U秩和检验。Spearman相关分析β-catenin蛋白表达与survivin蛋白表达的相关性。结果β-catenin蛋白在结直肠腺癌细胞膜、细胞浆及细胞核呈阳性表达,在老年癌组织组的阳性表达率显著高于正常组织组(64.0%vs 0.0%,P<0.001),其表达与性别无关(P>0.05),与淋巴结转移及TNM分期相关(P<0.05)。survivin蛋白在结直肠腺癌细胞核呈阳性表达,在老年癌组织组的阳性表达率显著高于正常组织组(54.0%vs 0.0%,P<0.001),其表达与性别、TNM分期无关(P>0.05),与淋巴结转移相关(P<0.05)。Spearman相关分析统计结果显示,老年结直肠腺癌患者的β-catenin蛋白表达与survivin蛋白表达呈显著正相关(r=0.645,P<0.001)。结论β-catenin和survivin蛋白均参与老年结直肠腺癌的发生发展,可能共同促进老年结直肠癌的进展。
Objective To detect the expression of β-catenin and survivin proteins in colorectal adenocarcinoma tissues of elderly patients, and investigate the correlation of their expression with gender, lymph node metastasis and tumor-lymph node-metastasis(TNM) stage. Methods A total of 50 colorectal adenocarcinoma patients who underwent surgical resection in our hospital from January 2015 to December 2017 were recruited in this study. The obtained specimens were divided into cancer tissue group(n=50) and normal tissue group(n=50, 2 cm away from the edge of the colorectal cancer mass, with microscopically normal tissue structure). The expression of β-catenin and survivin were detected by immunohistochemistry. SPSS statistics 22.0 was used for data analysis. Mann-Whitney U rank test was employed for comparison between 2 groups. Spearman test was applied to analyze the correlation of protein expression between β-catenin and survivin. Results β-catenin was mainly expressed in the membrane, cytoplasm and nucleus of colorectal adenocarcinoma cells, and its expression level was significantly higher in cancer tissues than normal tissues(64.0% vs 0.0%, P<0.05). Its expression was not related to gender(P>0.05), but was to lymph node metastasis and TNM stage(P<0.001). Survivin was located in the nuclei of colorectal cancer cells, and its expression was markedly higher in the colorectal cancer tissues than normal tissues(54.0% vs 0.0%, P<0.001). Its level had correlation with lymphatic invasion(P<0.05) but not with gender and TNM stage(P>0.05). Spearman correlation analysis showed that the protein expression of β-catenin was positively correlated to that of survivin in the elderly patients with colorectal adenocarcinoma(r=0.645, P<0.001). Conclusion Both of β-catenin and survivinare involved in the occurrence and development of colorectal cancer in elderly patients, and they may jointly promote its progression.
作者
常建兰
于俊岩
王宇
郭红亮
田向阳
刘平
李璐璐
周文雅
CHANG Jian-Lan;YU Jun-Yan;WANG Yu;GUO Hong-Liang;TIAN Xiang-Yang;LIU Ping;LI Lu-Lu;ZHOU Wen-Ya(1Department of Oncology, Affiliated Heping Hospital, Changzhi Medical College,Changzhi 046000, China;Department of Pathology, Affiliated Heji Hospital, Changzhi Medical College,Changzhi 046000, China)
出处
《中华老年多器官疾病杂志》
2019年第5期327-330,共4页
Chinese Journal of Multiple Organ Diseases in the Elderly
基金
国家自然科学基金(81800456)
长治医学院博士科研启动基金(BS15008)~~
