摘要
目的探讨MALAT1在鼻咽癌细胞细胞株中的表达及生物学功能。方法通过Real-time PCR检测MALAT1基因在鼻炎癌细胞株5-8F、C666-1、CNE-1、CNE-2、HONE-1、6-10B及永生化鼻咽上皮细胞NP69株中的表达;采用RNAi技术和RNA激活技术,构建MALAT1慢病毒干扰载体和激活载体载体并稳定转染鼻咽癌细胞株CNE-1,采用CCK8、平板克隆、划痕等试验分析MALAT1基因对CNE-1细胞生物学行为的影响。结果 MALAT1在高转移的鼻咽癌细胞株中高表达,在正常鼻咽上皮细胞低表达;经激活过表达MALAT1后,CNE-1细胞的上皮性标志物E-Cadherin的表达显著下调,间质细胞标志物Vimentin表达上调,侵袭转移能力明显增强(P<0.05)。结论 MALAT1基因上调能够促进鼻咽癌CNE-1细胞的增殖、侵袭和转移能力。
Objective To explore the expression of MALAT 1 in nasopharyngeal carcinoma （NPC） and its biological function. Method Real-time PCR was used to detect the expression of MALAT1 in 5-8F, C666-1, CNE-1, CNE-2, HONE-l, 6-10B and NP69 cell lines. CNE-1 cells engineered with MALAT1 RNA interference （RNAi） and RNA activation （RNAa） techniques were examined for changes in cell proliferation, invasion and metastasis using CCK8 assay, colony formation assay, TransweU in vitro invasion assay and wound-healing assay ability. Results MALAT1 was highly expressed in 5-8F cells with a high metastatic potential, and lowly expressed in normal nasopharyngeal epithelium cells. Overexpression of MALAT1 by RNAa suppressed the expression of E-cadherin, promoted the expression of vimentin and enhanced the proliferation, invasion, and metastasis of CNE-1 cells. Conclusion MALAT1 can enhance the proliferation, invasion, and metastasis of CNE-1 cells.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2013年第5期692-697,共6页
Journal of Southern Medical University
基金
国家自然科学基金(30770976
81172054)~~
关键词
MALAT1
鼻咽癌
基因表达
生物学功能
MALAT1
nasopharyngeal carcinoma
gene expression
biological significance
