摘要
目的探究梓醇对脑缺血-再灌注大鼠神经功能、氧化应激和炎症反应的影响。方法选取40只6周龄SPF级SD大鼠,采用改良线栓法制作脑缺血再灌注模型,并分为模型组、低浓度梓醇组(10 mg/kg)和高浓度梓醇组(20 mg/kg),另设空白对照组,每组10只。将其中低浓度梓醇组和高浓度梓醇组分别在术前3 d及术前30 min腹腔注射上述浓度的梓醇,空白对照组、模型组注射等量生理盐水。使用m NSS神经功能缺损评分评价大鼠神经功能;使用试剂盒检测大鼠脑组织丙二醛(MDA)、超氧化物歧化酶(SOD)、活性氧(ROS)和谷胱甘肽过氧化物酶(GPX)含量;使用ELISA检测白细胞介素1β(IL-1β)、白细胞介素6(IL-6)、肿瘤坏死因子α(TNF-α);使用试剂盒检测总一氧化氮合酶(t NOS)、诱导型一氧化氮合酶(i NOS)和一氧化氮(NO)的含量水平;使用Westen Bolt检测大鼠Bcl-2、Bax蛋白表达情况。结果 m NSS神经功能缺损评分结果显示,模型组评分显著高于高浓度梓醇组、低浓度梓醇组高于和空白对照组(P <0. 01);相比空白对照组,模型组MDA、ROS、IL-1β、IL-6、TNF-α、t NOS、i NOS、NO含量和Bax蛋白表达显著升高(P <0. 01); SOD、GPX含量和Bcl-2蛋白表达显著降低(P <0. 01)。相比模型组,低浓度梓醇组和高浓度梓醇组MDA、ROS、IL-1β、IL-6、TNF-α、t NOS、i NOS、NO含量和Bax蛋白表达显著降低,且高浓度梓醇组低于低浓度梓醇组,(P <0. 01); SOD、GPX含量和Bcl-2蛋白表达显著升高,且高浓度梓醇组高于低浓度梓醇组(P <0. 01)。结论梓醇可以有效缓解大脑缺血再灌注损伤,其作用机制与保护神经功能、降低氧化应激和炎症反应有关,且在一定浓度范围内呈浓度依赖性。
Objective To explore the effects of catalpol on neurological function,oxidative stress and inflammatory response in rats with cerebral ischemia-reperfusion.Methods A total of 40 6-week-old SPF SD rats were obtained.The middle cerebral artery occlusion models were established by modified suture method and were divided into four groups,including blank control group,model group,low-concentration catalpol group(10 mg/kg)and high-concentration catalpol group(20 mg/kg).Neurological function was assessed by the m NSS neurological deficit score.The levels of malondialdehyde(MDA),superoxide dismutase(SOD),reactive oxygen species(ROS)and glutathione peroxidase(GPX)were measured by reagent kit.The levels of interleukin-1β(IL-1β),interleukin-6(IL-6)and tumor necrosis factorα(TNF-α)in brain tissues were detected by ELISA.And the levels of total nitric oxide synthase(t NOS),inducible nitric oxide synthase(i NOS)and nitric oxide(NO)were detected by reagent kit.Western blot was used to detect protein expression levels of Bcl-2 and Bax in rats.Results The m NSS neurological deficit score in model group>high-concentration catalpol group>low-concentration catalpol group>blank control group(P<0.01).Compared with blank control group,the levels of MDA,ROS,IL-1β,IL-6,TNF-α,t NOS,i NOS and NO and Bax protein expression level in model group significantly increased(P<0.01).The levels of SOD and GPX and the Bcl-2 protein expression level significantly decreased(P<0.01).Compared with model group,the levels of MDA,ROS,IL-1β,IL-6,TNF-α,t NOS,i NOS,NO and Bax protein expression level significantly decreased in low-concentration catalpol group and high-concentration group(more remarkable in high-concentration group)(P<0.01).The levels of SOD,GPX and Bcl-2 protein expression level significantly increased in low-concentration catalpol group and high-concentration group(more remarkable in high-concentration group)(P<0.01).Conclusion Catalpol can effectively alleviate cerebral ischemia-reperfusion injury,and its mechanism is related to prot
作者
黄韬
孙静波
樊硕
成杰
HUANG Tao;SUN Jing-bo;FAN Shuo(Department of Pharmaceutical,Coal General Hospital,Beijing 100028,China.)
出处
《临床和实验医学杂志》
2019年第9期897-901,共5页
Journal of Clinical and Experimental Medicine
基金
河北省高等学校科学技术研究项目(编号:Z2017015)
关键词
大鼠
缺血再灌注
梓醇
神经功能
氧化应激
炎症反应
Rats
Ischemia-reperfusion
Catalpol
Neurological function
Oxidative stress
Inflammatory response
