摘要
为研究微生物群落在褐煤生物产气过程中的作用以及产气前后煤样性质的变化。以内蒙古胜利褐煤为产气底物,寺河矿区煤层气井排出水中富集产气微生物为出发菌群,在实验室开展褐煤生物产气实验,采用Illumina高通量测序平台分析产气前后微生物群落变化,并利用气相色谱、扫描电子显微镜等手段对褐煤产甲烷量和产气前后煤样物化性质及其煤表面的菌体形貌进行分析。结果表明,内蒙胜利褐煤可以被所富集得到的菌群利用并产生甲烷,产气周期为49 d,期间累计产甲烷量为83. 1 mL,净产甲烷率为7.84 mL/g煤。褐煤生物产气微生物样本中细菌群落多样性丰富,主要优势菌门为厚壁菌门(Firmicutes)、变形菌门(Proteobacteria)、WWE1、拟杆菌门(Bacteroidetes)、互养菌门(Synergistetes)和少量的脱铁杆菌门(Deferribacteres)。原始微生物群落结构多样性较高,经褐煤和基本培养基培养产气后,群落多样性降低。在微生物属水平上菌群结构变化较大,其中W22,Proteiniclasticum,VadinCA02,Tissierella_soehngenia,Clostridium, Dasulfovibrio等菌属在产气过程中发挥重要功能。内蒙胜利褐煤挥发分较高,富氢、富氧,煤表面结构松散有明显裂隙,有利于微生物附着降解,适宜进行生物产气试验。褐煤经微生物作用产气后,水分、灰分、挥发分均降低,固定碳百分比升高,H/C升高,S元素比例下降,利用扫描电镜观察到产气体系中煤表面附着大量短杆状和球状微生物,并存在类似微生物纳米导线的结构。
In order to study the role of microbial community in lignite biogas production process and the change of coal sample properties before and after gas production,the lignite obtained from Shengli in Inner Mongolia was used as the substrate for gas production,and the enriched anaerobic flora in Sihe mining area was used as the starting bacterial community.In the laboratory scale,the lignite biogas production was conducted.The microbial community changes before and after gas production were analyzed by Illumina high-throughput sequencing platform.Gas chromatography,scanning electron microscopy,etc.were used to analyze the methane production of lignite and the physicochemical properties of coal samples before and after gas production and the morphology of the coal surface.The results showed that Inner Mongolia Shengli lignite could be utilized by the enriched flora and produce methane with a biogas production cycle of 49 days,and the cumulative methane production was 83.1 mL.The net methane production rate was 7.84 mL/g coal.The diversity of bacterial communities in the biomass biogenic samples of lignite was abundant.The main dominant bacteria at phylum level were Firmicutes,Proteobacteria,WWE1,Bacteroidetes,Synergistetes and Deferribacteres.The initial microbial flora had high diversity,however,the microbial diversity decreased after the biogas generation with lignite and basic medium.The microbial structure changed obviously at genus level,and the dominant bacteria W22,Proteiniclasticum,VadinCA02,Tissierella soehngenia,Clostridium and Desulfovibrio played important roles in the biogas generation.Inner Mongolia Shengli lignite had higher volatile matter,rich in hydrogen and oxygen,and the surface structure of coal was loose,with obvious cracks,which was beneficial to microbial adhesion and degradation,and was suitable for biogas production.After the biogas production,lignite’s volatile content,water and ash,the proportion of S and O elements decreased,however the percentage of fixed carbon and the H/C increased.Th
作者
赵晗
何环
王江泽
谭凯丽
赵娜
任恒星
ZHAO Han;HE Huan;WANG Jiangze;TAN Kaili;ZHAO Na;REN Hengxing(State Key Laboratory of Coal and CBM Co-mining,Shanxi Jincheng Anthracite Mining Group Co.,Ltd.,Jincheng 048000,China;School of Chemical Engineering and Technology,China University of Mining and Technology,Xuzhou 221116,China;Key Laboratory of Coal Processing and Efficient and Clean Utilization of Ministry of Education,China University of Mining and Technology,Xuzhou 221116,China;Yi’an Lanyan Coal and Coal-bed Methane Simultaneous Extraction Technology Co.,Ltd.,Jincheng 048000, China)
出处
《煤炭学报》
EI
CAS
CSCD
北大核心
2019年第4期1224-1231,共8页
Journal of China Coal Society
基金
山西省煤层气联合研究基金资助项目(2016012009)
中央高校基本科研业务费专项资金资助项目(2017XKQY037)
关键词
褐煤
生物产气
群落变化
高通量测序
lignite
biogenic gas
microbial flora succession
high-throughput sequencing
