摘要
采用气相色谱分析、扫描电子显微镜分析和红外光谱分析等方法,研究了锯末与煤的质量比对产甲烷周期以及生物气组成的影响,确定了锯末与煤增产甲烷的最佳质量比。结果表明:驯化后的菌源主要由细菌(相对丰度为91.67%)和古菌(相对丰度为8.33%)组成;锯末与煤的质量比对产甲烷周期有影响,当锯末与煤的质量比分别为0∶1,0.5∶1,1∶1,1.5∶1时,产甲烷第一阶段为快速阶段(0 d~7 d),第二阶段为慢速阶段(7 d~28 d),而当锯末与煤的质量比为2∶1时,产甲烷的第一阶段是慢速阶段(0 d~14 d),第二阶段是快速阶段(14 d~28 d);在微生物降解煤产气过程中,添加适量的锯末可提高甲烷产量,但锯末过量会抑制甲烷产量,在锯末与煤的质量比分别为0.5∶1,1∶1,1.5∶1的条件下,扣除空白组(不含锯末和煤的混合物)甲烷产量(378.15μmol/g)后,锯末与煤共降解的甲烷产量分别为303.35μmol/g,385.21μmol/g,303.94μmol/g,比煤单独降解的甲烷产量(268.83μmol/L)分别增加了12.84%,43.29%和13.05%,而当锯末与煤的质量比为2∶1时,扣除空白组甲烷产量后,锯末与煤共降解的甲烷产量为95.19μmol/g,比煤单独降解的甲烷产量减少了64.59%。扫描电子显微镜结果表明,不同锯末和煤质量比对煤的降解程度影响不同。红外光谱分析发现,添加一定量的锯末后,会增加样品中碳碳双键含量,降低芳烃和羧基含量。一定量的锯末可提升微生物对煤的利用效率,增加生物甲烷产量,该方法可为增产生物甲烷量提供一种新思路。
Gas chromatography,scanning electron microscopy and infrared spectroscopy methods were used to explore the effects of the mass ratio of sawdust and coal on the composition of biogas and methane production and the optimal mass ratio of sawdust to coal was determixed to increase methane production.Results show that the bacterial source after domestication is mainly composed of bacteria(relative abundance is 91.67%)and archaea(relative abundance is 8.33%),the different mass ratios of sawdust and coal affect the methane production cycle.When the mass ratios are 0.5∶1,1∶1,and 1.5∶1,the first stage of methane production is a fast stage(0 d-7 d),and the second stage is a slow stage(7 d-28 d).When the mass ratio is 2∶1,the first stage of methane production is a slow stage(0 d-14 d),and the second stage is a fast stage(14 d-28 d).In addition,during the microbial degradation of coal for gas production,appropriate proportion addition of sawdust can improve the methane production.Excessive sawdust will inhibit the methane production.When the mass ratios of sawdust to coal are 0.5∶1,1∶1,and 1.5∶1,respectively,the methane productions of sawdust and coal co-degradation are 303.35μmol/g,385.21μmol/g,and 303.94μmol/g after subtracting the blank group(378.15μmol/g),corresponding to an increase of 12.84%,43.29%,and 13.05%compared with the degradation with coal alone.When the mass ratio of sawdust to coal is 2∶1,the methane produced from the co-degradation of sawdust and coal is 95.19μmol/g,which is reduced by 64.59%compared with the degradation of coal alone.Furthermore,electron microscopy results show that different sawdust and coal mass ratio have different effects on coal degradation.The infrared spectroscopy results show that adding a certain amount of sawdust will increase the content of carbon-carbon double bond in the sample and decrease the contents of aromatic hydrocarbons and carboxyl groups.A certain amount of sawdust can improve the microbial utilization efficiency of coal and increase biomethane pr
作者
武俐
李啸林
曹斌
赵同谦
刘兴勇
WU Li;LI Xiaolin;CAO Bin;ZHAO Tongqian;LIU Xingyong(College of Resources and Environment,Henan Polytechnic University,454000 Jiaozuo,China;Collaborative Innovation Center for Coal-bed Methane and Shale Gas of Henan,Henan Polytechnic University,454000 Jiaozuo,China)
出处
《煤炭转化》
CAS
CSCD
北大核心
2023年第2期20-27,共8页
Coal Conversion
基金
国家自然科学基金项目(41702174)
河南省重点研发与推广专项项目(222102320213)
河南理工大学博士基金项目(B2021-74).
关键词
生物甲烷
增效
锯末
低阶煤
微生物降解
biomethane
increasing efficiency
sawdust
low-rank coal
microbial degradation
