摘要
为研究岩扇贝Crassadoma gigantean的生物学特性和消化代谢机能,采用传统培养方法分离培养加拿大原种岩扇贝内脏团(JN)和肠道(JC)及中国育种得到的子一代岩扇贝内脏团(YN)和肠道(YC)4种样品中的细菌,测定分离到的可培养细菌的16S rRNA基因并确定其分类地位;提取上述样品中的总DNA,运用Ion S5^(TM)XL高通量测序平台,对样品中细菌群落多样性进行分析。结果表明:用传统方法获得可培养菌株83株,选取代表菌31株测序并比对,其分别属于嗜冷杆菌属Psychrobacter、动性球菌属Planococcus、弧菌属Vibrio、假单胞菌属Pseudomonas、假交替单胞菌属Pseudoalteromonas、芽孢杆菌属Bacillus、库克菌属Kocuria和副球菌属Paracoccus;高通量测序结果显示,4种样品中有效序列群落结构可分为9个门,分别为变形菌门Proteobacteria、异常球菌-栖热菌门Deinococcus-Thermus、拟杆菌门Bacteroidetes、厚壁菌门Firmicutes、蓝细菌门Cyanobacteria、软壁菌门Tenericutes、放线菌门Actinobacteria、浮霉菌门Planctomycetes和疣微菌门Verrucomicrobia;加拿大原种岩扇贝肠道样品中的优势菌门为变形菌门(占80%),次优势菌门为拟杆菌门(占15%),内脏团样品中的优势菌门为变形菌门(占52%),次优势门为拟杆菌门(占18%);中国育种得到的子一代岩扇贝肠道样品中的优势菌门为变形菌门(占61%),次优势菌门为拟杆菌门(占26%),内脏团样品中的优势菌门为变形菌门(占73%),次优势菌门为拟杆菌门(占17%)。研究表明,岩扇贝的肠道和内脏团微生物具有丰富的多样性,加拿大原种岩扇贝和国内子一代岩扇贝的肠道和内脏团细菌群落结构存在一定差异。
In this paper, bacteria were isolated and cultured from viscera and digestive tract of Canadian rock scallop Crassadoma gigantea and the first generation of congener rock scallop from artificial breeding in China by the traditional method, and bacterial community diversity was analyzed by 16 S rRNA identification, and total DNA using Ion S5TMXL high throughput sequencing platform. The results showed that 83 strains of culturable strains were found by the traditional method, and 31 strains of representative strains were sequenced and compared,including Psychrobacter, Planococcus, Vibrio,Pseudomonas, Pseudoalteromonas, Bacillus, Kocuria and Paracoccus. The high throughput sequencing indicated that the effective sequence community structure of the 4 samples was divided into 9 phyla: Proteobacteria, Deinococcus-Thermus, Bacteroidetes, Firmicutes, Cyanobacteria,Tenericutes, Actinobacteria, Planctomycetes, and Vercomicrobia, with dominant Proteobacteria in the intestines of Canadian rock scallops, accounting for 80%, and the subdominant Bacteroidetes, accounting for 15%. There were dominant Proteobacteria, accounting for 52%, and the subdominant Bacteroidetes, accounting for 18%, in the viscera of Canadian rock scallops. The dominant phylum Proteobacteria, accounting for 61%, and subdominant phylum Bacteroides, accounting for 26%, were observed in the intestine of the subgeneration rock scallops bred in domestic. The dominant phylum Proteobacteria, accounting for 73%, and the subdominant phylum Bacterioids, accounting for 17%, in the viscera in the first generation rock scallops. The findings indicated that there was rich in microorganism diversity in intestine and viscera of rock scallops, with to some extent, differences between Canadian rock scallop and subgeneration rock scallops bred in China.
作者
邹建威
曹善茂
李晓雨
刘钢
王尧
王斌
ZOU Jian-wei;CAO Shan-mao;LI Xiao-yu;LIU Gang;WANG Rao;WANG Bin(Key Laboratory of Marine Bio-resources Restoration andHabitat Reparation in Liaoning Province, Dalian OceanUniversity, Dalian 116023,China)
出处
《大连海洋大学学报》
CAS
CSCD
北大核心
2019年第3期323-330,共8页
Journal of Dalian Ocean University
基金
辽宁省海洋与渔业厅项目(201214)
辽宁省教育厅科研项目(L201624)
关键词
岩扇贝
肠道菌群
内脏团菌群
多样性
Crassadoma gigantea
intestinal microorganism
visceral microorganism
diversity
