摘要
研究果树抗寒机理及其抗寒生理机制对于抗寒基础理论研究,以及解决实际生产上的问题十分重要。为了了解核桃相关抗寒基因的表达机制,本研究采用2、4、6、8℃几个低温进行处理,利用实时荧光定量PCR技术研究了在不同温度下‘阳光’‘云新’核桃叶片内CBF、(GenBank序列号:JX875914.1)随时间表达量的变化。本课题从处理后核桃叶片中提取出了c-DNA,并成功得到了实时荧光定量PCR数据并进一步进行比较分析。为了了解核桃相关抗氧化生理机制,采用分光光度比色法研究了核桃叶片的多酚、黄酮成分对低温胁迫响应的规律,并得到了相关抗寒物质的变化规律,旨在为以后选育核桃优良抗寒品种等提供更多的参考依据。1.本课题以核桃18S基因为内参,对‘阳光’和‘云新’核桃内CBF基因在2、4、6、8℃胁迫下的表达进行了初步的研究,表明当受到冷胁迫时这些基因在核桃叶片内均有表达量的变化,其中‘阳光’核桃叶片受到冷胁迫时这种变化更为明显,表现为‘阳光’核桃在6℃胁迫下出现了先升高后下降再升高下降的趋势,在8℃胁迫下出现了先升高后下降的明显趋势,推测CBF基因在核桃对低温胁迫的响应中发挥重要功能。本研究同时表明‘阳光’核桃对冷胁迫更敏感,更能在寒冷胁迫迅速响应。
Studying the molecular mechanism of cold resistance in fruit trees will contribute to the understanding of plant stress response and also the agriculture industry.In order to understand the effect of polyphenols and flavone in the response to cold stress under various conditions(at the temperature 2、4、6、8℃)RT-PCR was used to examine the expression of CBF gene(GenBank:JX875914.1)of two different species Juglans leaves.Moreover,we studied the response of polyphenols and flavonoids to the low temperature stress by spectrophotometer.Using Juglans 18S gene as a reference,we observed that the expression of CBF gene were changed in Walnut Leaves under cold stress,and the expression fluctuation is more dramatic in the‘Yangguang’Walnut Leaves under cold stress.All of the three genes in the‘Yangguang’Walnut leaf at 6℃first increased and then decreased,followed by a trend of increasing and then decreasing,whereas the expression of the genes rises at first and then decrease at 8℃.Thus we propose that the three genes play important roles in the response of Juglans to low temperature stress.
作者
宋静武
彭磊
Song Jingwu;Peng Lei(Zigui County Forestry Bureau Zigui County Forestry Science and Technology Promotion Center Yichang 443600;College of horticulture, yunnan agricultural university, kunming 650201)
出处
《湖北林业科技》
2019年第2期7-13,共7页
Hubei Forestry Science and Technology
关键词
核桃
CBF
抗逆性
机制
寒冷胁迫
Juglans
C-repeat binding factors
stress resistance
principle
cold stress
