摘要
目的探讨不同剂量米非司酮对大鼠子宫内膜异位症的疗效及对病灶中环氧合酶-2(COX-2)、血管内皮生长因子(VEGF)表达的影响。方法采用在位子宫内膜局部种植的方法制造子宫内膜异位症大鼠模型,将112只成功造模大鼠随机分为对照组,米非司酮低剂量组、中剂量组、高剂量组。各组以不同浓度米非司酮灌胃4周后比较病灶体积,并取异位子宫内膜组织检测COX-2、VEGF mRNA水平。结果造模成功后异位内膜组织中COX-2、VEGF mRNA的表达水平较造模前在位内膜组织中COX-2、VEGF mRNA的表达明显升高(P<0.05)。与对照组比较,米非司酮治疗组大鼠异位病灶体积均缩小,其中高剂量组更明显(P<0.05);不同剂量治疗组异位内膜组织COX-2、VEGF mRNA表达均较对照组下降(P<0.05),高剂量组更加明显(P<0.05)。COX-2mRNA和VEGF mRNA的表达呈正相关(r=0.782,P<0.05)。结论米非司酮可有效改善大鼠子宫内膜异位症症状,其作用机制可能通过下调大鼠子宫内膜异位病灶中COX-2、VEGF表达,抑制病灶的进展,其作用呈浓度依赖性。
Objective To discuss the curative effect of different doses of mifepristone on rat endometriosis and its influence on expression of cyclooxygenase-2(COX-2)and vascular endothelial growth factor(VEGF)in lesions.Methods The endometriosis rat model was made by adopting the endometrial local implantation.A total of 112 successfully model-constructing rats were randomly divided into the control group,low mifepristone dose group,middle mifepristone dose group and high mifepristone dose group.Each group was given the gavage by different concentrations of mifepristone for 4 weeks.Then the endometriosis tissue was taken for detecting the expression levels of COX-2 and VEGF mRNA.Results After successfully modeling,the expression levels of COX-2 mRNA and VEGF mRNA in ectopic endometrial tissue were significantly increased compared with those before modeling(P<0.05).Compared with the control group,the volume of ectopic lesions in the mifepristone treatment group was all reduced,which in the high dose group was more obvious(P<0.05).The COX-2 and VEGF mRNA expressions of ectopic endometrial tissue in different doses of group was decreased compared with the control group(P<0.05),while which in the high dose group was more obvious(P<0.05).CCOX-2 mRNA was positively correlated with VEGF mRNA expression(r=0.782,P<0.05).Conclusion Mifepristone can effectively improve the symptoms of rat endometriosis.Its mechanism may down-regulate the expression of COX-2 and VEGF in endometriosis lesion and inhibit the lesion progress,and its role shows the concentration dependence.
作者
南燕
李玉洁
NAN Yan;LI Yujie(Department of Gynaecology and Obstetrics,the Third Affiliated Hospital of Xinxiang Medical University,Xinxiang,Henan 453003,China)
出处
《重庆医学》
CAS
2019年第6期942-944,948,共4页
Chongqing medicine
基金
河南省教育厅科技攻关项目(122102310490)
