摘要
为了解稻瘟菌无毒基因AvrPii在水稻病原菌相关分子模式诱导的免疫反应(PTI)抗病分子信号调控中的功能,对AvrPii基因编码蛋白的分子结构特性进行分析,构建了AvrPii基因的植物表达载体幵进行遗传转化。结果表明:AvrPii蛋白由70个氨基酸组成,相对分子质量约为7 500,等电点为6.0,总平均亲水性值为–0.159;AvrPii蛋白包含1个信号肽、1个跨膜结构域和2个基序为LxAR和CxxCxxxxxxxxxxxH的保守结构域;利用农杆菌侵染转化法,获得AvrPii基因全长序列和不含信号肽AvrPiinsp的转基因株系13个,PCR扩增和实时荧光定量PCR鉴定结果表明,AvrPii和AvrPiinsp基因已成功转入受体品种日本晴幵得到稳定表达。
To characterize the biological function of AvrPii involved in rice PAMPs-triggered immunity(PTI) immune signaling, the properties of AvrPii protein encoded by AvrPii were analyzed, and the transgenic rice with AvrPii gene was constructed. The results showed that AvrPii protein is composed of 70 amino acids with a molecular weight of 7 500, the theoretical isoelectric point is 6.0 and the average value of hydrophilic amino acids is-0.159. The AvrPii protein includes one signal peptide, one transmembrane domain, and two conserved motif with residues LxAR and CxxCxxxxxxxxxxxH. Thirteen transgenic lines with full length AvrPii gene or AvrPiinsp gene without the signal sequence were obtained. PCR detection and expression analysis confirmed the insertion and the transcriptional expression of AvrPii and AvrPiinsp in the receptor cultivar Nipponbare.
作者
周小龙
符策纠
李冰
丁晓雯
林晋军
刘雄伦
刘釐灵
ZHOU Xiaolong;FU Cejiu;LI Bing;DING Xiaowen;LIN Jinjun;LIU Xionglun;LIU Jinling(College of Agronomy,Hunan Agricultural University,Changsha,Hunan 410128 China;Hunan Provincial Key Laboratory of Rice and Rapeseed Breeding for Disease Resistance,Changsha,Hunan 410128,China;Southern Regional Collaborative Innovation Center for Grain and Oil Crops in China,Changsha ,Hunan,410128 China)
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2019年第2期118-123,共6页
Journal of Hunan Agricultural University(Natural Sciences)
基金
国家自然科学基釐项目(31401726)
湖南省自然科学基釐项目(2015JJ3079)
湖南农业大学人才计划和作物学科开放基釐项目(14YJ01
ZWKF201503)
