摘要
大豆蛋白中蛋氨酸等含硫氨基酸含量偏低,探究蛋氨酸合成过程关键酶胱硫醚-γ-合成酶编码基因Gm CGS表达规律,对于改善这一缺陷具有十分重要的意义。本研究以过表达At D-CGS转基因大豆株系及其受体品种Jack为材料,从上游物质硫酸根和下游产物蛋氨酸两方面入手,探究硫酸根和蛋氨酸对Gm CGS在转录水平上的影响。结果显示,较高浓度的硫酸根会促进野生型材料中该基因的表达,抑制转基因材料中该基因的表达;蛋氨酸处理之后,对Gm CGS的表达没有显著影响;转基因株系中,Gm CGS表达量显著低于野生型材料中该基因表达量。以上结果说明通过外界硫肥等栽培措施的改变只可以小幅度改变Gm CGS表达量,难以有效改善大豆蛋氨酸含量偏低的缺陷,这为提高大豆蛋氨酸含量提供了一定的理论参考。
Due to low content of sulfur containing amino acid in soybean,understanding of cystathionine gamma-synthase coding gene GmCGS which plays a key role in synthesis of methionine,at transcriptional level is important to solving this problem.In this study,the transcriptional level of CGS in Transgenic soybean lines overexpressing AtD-CGS and wild type (WT) was detected after sulfate or methionine treatment designed to identify their effects on regulating the expression of GmCGS.After treated with 4.5 mmol/L sulfate,the relative expression level of GmCGS was significantly increased in WT,whereas the transcription level of GmCGS in transgenic lines overexpressing AtD-CGS was significantly decreased.No significant difference was observed after methionine treatment.Compared to WT,the transcriptional level of GmCGS was significantly decreased in transgenic lines.The results indicated that the effect of cultivation technique on expression of GmCGS was too limited to improve methionine content.All of these provided reference for increasing content of Met in soybean.
作者
刘修杰
陈莉
郭晨
蔡宇鹏
孙石
韩天富
侯文胜
LIU Xiu-jie;CHEN Li;GUO Chen;CAI Yu-peng;SUN Shi;HAN Tian-fu;HOU Wen-shen(Institute of Crop Sciences,Chinese Academy of Agricultural Sciences/National Center for Transgenic Research in Plants,Beijing 100081,China;Institute of Crop Sciences,Chinese Academy of Agricultural Sciences/Ministry of Agriculture Key Laboratory of Soybean Biology (Beijing),Beijing 100081,China)
出处
《中国油料作物学报》
CAS
CSCD
北大核心
2019年第1期10-17,共8页
Chinese Journal of Oil Crop Sciences
基金
国家重点研发项目(2016YFD0100504)
国家自然科学基金(31471571)
国家转基因生物新品种培育重大专项(2016ZX08004-003
2016ZX08010-004)
