摘要
无菌采集病死鸡的肝脏、肾脏、脾脏、肺脏等组织,接种于麦康凯平板和普通琼脂,进行细菌分离与培养,并对分离菌进行生化试验和16s rDNA基因序列分析,同时用14种抗菌药物对分离菌株进行药敏试验。结果显示,分离菌在麦康凯平板上呈红色圆形菌落;镜检结果为革兰氏阴性杆菌;分离菌株能够发酵葡萄糖、棉籽糖、乳糖、木糖等糖类并产酸产气,鸟氨酸及赖氨酸试验阳性,苯丙氨酸脱氨酶及硫化氢阴性,符合大肠杆菌的生化特性;16s rDNA基因序列测定结果显示分离菌株为大肠杆菌;分离菌株对菌必治、美洛西林、恩诺沙星、诺氟沙星的敏感率较高,而对萘啶酸、青霉素等耐药。本研究为鸡大肠杆菌的诊断和防治提供了依据。
Livers, kidneys, spleens, lungs and other tissues of dead chickens were inoculated onto MacConkey plates and common agar plates for bacterial isolation. The biochemistry reactions, PCR amplification for 16s rDNA were used to identifying the obtained isolates. As a result, one isolate was obtained, which had red round colonies on MacConkey plates and was Gram-negative in microscopic examination. Results showed that the isolate fermented glucose, raffinose, lactose and xylose but did not produce hydrogen sulfide. Meanwhile, 16s rDNA sequencing indicated that this isolate was E. coli. Additionally, the drug susceptibility test showed that this isolate was highly sensitive to ceftriaxone, mezlocillin, norfloxacin and enrofloxacin but resistant to nalidixan, penicillin and trimethoprim.
作者
江林宜
JIANG Lin-yi(Bailu Animal Epidemic Prevention Quarantine, Guannan Country, Lianyungang City, Jiangsu Province, Guannan 223500, China)
出处
《中国动物传染病学报》
CAS
北大核心
2019年第1期100-103,共4页
Chinese Journal of Animal Infectious Diseases
关键词
大肠杆菌
耐药性
分离
鉴定
Escherichia coli
resistance
isolation
identification
